The aim of this study was to evaluate the toxic effect of glyphosate (5,33 μg/ml, 0,53 μg/ml and 0,53 μg/ml), terbuthylazine (26,5 μg/ml, 2,65 μg/ml i 0,00366 μg/ml), and the mixture of active compounds, on human peripheral blood lymphocytes, by in vitro examination using micronucleus assay. Results indicate that glyphosate, terbuthylazine and their mixture caused increased formation of micronuclei, nuclear buds and nucleoplasmic bridges as well as the apoptosis and necrosis. Although statistical significance was observed in the samples treated with the highest concentration of glyphosate (5,33 μg/ml), terbuthylazine (26,5 μg/ml) and their mixture, increase of nucleoplasmic bridges was not significant for any of used concentrations. Results obtained indicate that the majority of damages in treated lymphocytes were induced by combination of clastogenic and aneugenic effect of glyphosate and terbuthylazine, which is shown in the results of the mixture of active compounds. Results obtained with separate treatment of lymphocytes with either glyphosate or terbuthylazine, showed no effect on pericentromeric regions of chromosomes 1, 9, 15 and 16. However, results obtained after treatment with terbuthylazine showed its potential specific effect on nucleolus organizing regions. Glyphosate showed no effect on the cell cycle kinetics while in the presence of terbuthylazine the kinetics was retarded. In the mixture of active compounds terbuthylazine showed stronger effect on the cell cycle kinetics.