Posljednjih nekoliko godina eutektična se otapala intenzivno proučavaju kao zelena zamjena za sveprisutna tradicionalna škodljiva otapala u procesima organske sinteze i biokatalize te stoga privlače znatnu pozornost unutar biotehnološke i kemijske industrije. Biokataliza se pokazala kao ekološki prihvatljiva alternativa kemijskim katalizama, a posebna pozornost se posvećuje biokatalizi u nevodenim otapalima. U okviru ovog rada ispitana je redukcija etil-acetoacetata u etil-3-hidroksibutanoat u kolinijevim eutektičnim otapalima s monosaharidom kao donorom vodika primjenom pekarskog kvasca Saccharomyces cerevisiae kao biokatalizatora.

In recent years, deep eutectic solvents have been intensively studied as a possible replacement for conventional molecular solvents for biocatalytic and organic reactions and have been the subject of considerable attention within chemical technology and biotechnology. Biocatalysis proved as environment friendly alternative to chemical catalysis and special attention has been devoted to biocatalysis in non-aqueous medium. Within this work, reduction of etyl acetoacetate to ethyl-3-hydroxybutanoate has been tested with a use of baker’s yeast Saccharomyces cerevisiae as a biocatalyst in cholinium-based deep eutectic solvents with monosaccharide as hydrogen bond donor.