Intracellular trafficking of pDNA. Cytosolic migration and nuclear import

diplomski rad

• Autor: Martina Živković
• Mentor: Vladimir Mrša (mentor)
• Ustanova koja je dodijelila akademski / stručni stupanj: Sveučilište u Zagrebu; Prehrambeno-biotehnološki fakultet; Zagreb
• Datum i država obrane: 2014-07-04, Hrvatska
• Znanstveno / umjetničko područje, polje i grana: BIOTEHNIČKE ZNANOSTI; Biotehnologija

Sažetak

Gene therapy provides a great hope for the treatment of many acquired and inherited life-threatening diseases. Nucleic acid drugs, however, must be delivered to the interior of the target cell while surviving an array of biological barriers. Successful gene therapy is thus dependent on the development of an efficient delivery vector. Nonviral vectors, as an attractive alternative to viral vectors, have been intensively investigated for decades; advantages such as a potentially improved safety profile, flexibility in the size of the delivered gene and lower immunogenicity have all been demonstrated. One of the most critical biological barriers for successfully delivery of pDNA with non-viral vectors is a cytoplasmic transport and nuclear import. In this work, to overcome these barriers, I performed the transfection of different cell lines using polyplexes and lipoplexes combined with two signals which allow easier cytoplasmic trafficking and nuclear import of pDNA. For the successful delivery of pDNA towards the nucleus, we investigated the possibility of peptide P79-98 for docking pDNA onto microtubules. For nuclear import we explored the capacity of 3NF sequence inserted in pDNA which is recognized by the transcription factor NFκB and shuttled into nucleus.

• Ključne riječi: gene therapy; transfection; polyplexes; lipoplexes; microtubules; nuclear import
• Jezik: engleski
• URN:NBN: urn:nbn:hr:159:370880
• Studijski program: Naziv: Molekularna biotehnologija; Vrsta studija: sveučilišni; Stupanj studija: diplomski; Akademski / stručni naziv: magistar/magistra inženjer/inženjerka molekularne biotehnologije (mag. ing. biotechn.)
• Vrsta resursa: Tekst
• Način izrade datoteke: Izvorno digitalna
• Prava pristupa: Pristup korisnicima matične ustanove
• Datum i vrijeme pohrane: 2017-04-28 11:42:15