Determination of quinolone residues in muscle and eggs samples by ELISA method

master's thesis

• Title: Određivanje rezidua kinolona u uzorcima mišića i jaja ELISA metodom
• Author: Katarina Jančec
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2012-10-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kinoloni su sintetski antibiotici širokog spektra djelovanja. Njihova široka primjena u proizvodnji hrane zabrinjavajuća je zbog postojećih dokaza o negativnom utjecaju na gustoću kostiju kod djece i trudnica, neurotoksičnosti i mogućih dermatoloških reakcija. Prisutnost rezidua veterinarskih lijekova u namirnicama životinjskog porijekla stvorila je potrebu njihova dokazivanja u mesu, mlijeku, jajima i drugim supstratima. Poznato je više metoda dokazivanja prisutstva i količine lijekova u namirnicama životinjskog porijekla od kojih je ELISA test brzi test, jednostavan za izvođenje i dizajniran za paralelno određivanje velikog broja uzoraka, a postupci su vrlo osjetljivi i daju pouzdane rezultate. Svrha rada je određivanje rezidua kinolona u nasumičnim uzorcima mesa i jaja prikupljenim na području Republike Hrvatske. Provedeno je određivanje na 608 uzoraka (408 uzoraka mišića i 200 uzoraka jaja). Analizom je dobiveno 6 sumnjivih uzoraka mišića i 19 sumnjivih uzoraka jaja. Uzorci kod kojih se sumnjivi rezultat dobije tri puta analiziraju se potvrdnom LC-MS metodom.

Abstract (english)

Quinolones are synthetic antibiotics having broad spectrum of activity. Their widespread usage in food production is a concern because of existing evidence about the negative impact on bone density in children and pregnant women, and possible neurotoxicity dermatologic reactions. . The presence of veterinary drug residues in food of animal origin has created a need to prove them in meat, milk, eggs and other substrates. It is known more than one method of proving the presence and quantity of drugs in food of animal origin. Among them the ELISA test is rapid, easy to perform and is designed for parallel determination of a large number of samples. The procedure is also very sensitive and gives reliable results. The goal of this work is determination of quinolone residues in random meat and eggs samples collected on the Croatian territory, and was performed on 608 samples (408 samples of muscles and 200 samples of eggs). The analysis proved that 6 muscle samples and 19 eggs samples were suspicious. Samples in which the suspicious result is obtained three times has to be analyzed by LC-MS confirmation method.

• Keywords: veterinarsko-medicinski lijekovi; kinoloni; ELISA test
• Keywords (english): veterinary-medical drugs; quinolones; ELISA test
• Language: croatian
• URN:NBN: urn:nbn:hr:159:582390
• Study programme: Title: Biotehnologija; smjerovi: Biokemijsko-mikrobiološki; Study programme type: sveučilišni; Study level: dodiplomski; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Restricted access
• Created on: 2017-05-17 09:18:21