Optimiranje parametara za određivanje dvokomponentnog analita metodom omjera spektrofotometrijskih signala

master's thesis

• Title (english): Optimization of parameters for spectrophotometric determination of two-component analytes using signals ratio method
• Author: Ivana Pranjić
• Mentor: Zorana Grabarić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for General and Inorganic Chemistry and Electroanalysis); Zagreb
• Defense date and country: 2012-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Za kvantitativno određivanje sintetskih azo-bojila Sunset Yellow i Azorubine u binarnim smjesama, u ovom radu, korištena je metoda omjera spektrofotometrijskih signala. U cilju optimiranja metode ispitivan je utjecaj različitih parametara na rezolviranje globalnog signala i određivanje pojedinih komponenata u sintetskim binarnim smjesama i uzorku arome jagode uz poznati dodatak jedne komponente. Uz tako dobivene optimalne parametre određena je koncentracija prehrambenih bojila u uzorku voćnog soka. Rezultati dobiveni metodom omjera signala uspoređeni su s rezultatima derivacijske metode te su pokazali slaganje u rasponu do 10 %.

Abstract (english)

The signals ratio spectrophotometric method was applied in this work for simultaneous quantitative determination of food dyes Sunset Yellow and Azorubine. In order to optimize the signals ratio method, the effect of different parameters on the accuracy of the obtained results was investigated in binary mixtures and semi-real sample and optimal parameters were then applied for determination of the concentration of dyes in a sample of fruit juice. The concentrations obtained for individual dyes in binary synthetic mixtures and real samples were compared with the results obtained using derivative spectroscopy. Results obtained using the ratio of the signal are compared with the derivative method and results showed agreement in the ranging up to 10%.

• Keywords: sintetska bojila; spektrofotometrija; metoda omjera signala; derivacijska spektrofotometrija; voćni sok.
• Keywords (english): synthetic dyes; spectrophotometry; signals ratio method; derivative spectrophotometry; fruit juice
• Language: croatian
• URN:NBN: urn:nbn:hr:159:004324
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-17 13:04:43