Hepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša

master's thesis

• Title (english): Hepatoprotective effect of polyphenols present in blackthorn extract on antioxidative status in mice
• Author: Ivona Paradžik
• Mentor: Irena Landeka Jurčević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Chemistry and Biochemistry); Zagreb
• Defense date and country: 2016-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Cilj ovog rada bio je ispitati antioksidativni utjecaj polifenola ekstrakta cvijeta trnine (Prunus spinosa L.) i visoko-proteinske dijete (whey protein) na markere oksidacijskog stresa u jetri C57BL6 miša. Ispitivanje je provedeno kroz 30 dana, pri čemu su životinje bile podijeljene u 4 grupe, ovisno o vrsti tretmana: 1. grupa- kontrolna, 2. grupa- TC (tretirane ekstraktom cvijeta trnine), 3. grupa- WP (tretirane whey proteinom), 4. grupa- WP+TC. Karbonilirani proteini (PC), reducirani glutation (GSH), malondialdehid (MDA), superoksid dismutaza (SOD) te katalaza (CAT) su biomarkeri oksidacijskog stresa praćeni u ovom istraživanju. U odnosu na kontrolnu skupinu životinja, tretman s TC statistički je značajno (ANOVA, p<0,05) smanjio aktivnosti MDA i PC u jetri miša. Takav rezultat pripisuje se prisutnim polifenolima u ekstraktu cvijeta trnine. U odnosu na kontrolnu skupinu životinja, koncentracije SOD, GSH i CAT su u svim grupama životinja statistički značajno (ANOVA, p<0,05) povećane, što znači da se oksidacijski stres smanjio. Takav rezultat posljedica je antioksidacijskog učinka i ekstrakta trnine i whey proteina. Istraživanjem se utvrdilo da ekstrakt cvijeta trnine sadrži mnogo bioaktivnih spojeva koji, u kombinaciji sa primjenom visoko-proteinske dijete (whey protein), imaju povoljan utjecaj na smanjenje oksidacijskog stresa i povećanje aktivnosti endogenih antioksidacijskih enzima.

Abstract (english)

The aim of this study was to examine antioxidant effect of polyphenols from blackthorn (Prunus spinosa L.) extract and high-protein (whey protein) diet on markers of oxidative stress in the liver of C57BL6 mice. Testing obtained for 30 days and the animals were divided into 4 groups, depending on the type of treatment: group 1- control, group 2- TC (treated with blackthorn flower extract), group 3- WP (treated with whey protein) and group 4 - WP+TC. Carbonylated proteins (PC), reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were biomarkers of oxidative stress monitored in this study. Compared to the control group of animals, treatment with TC statistically significant (ANOVA, p<0,05) reduced the activity od MDA and PC in the liver of mice. The result is attributed to the polyphenols present in the extract of a blackthorn flower. Compared to the control group of animals, concentrations of SOD, CAT and GSH in all groups of animals were statistically significant (ANOVA, p<0,05) increased, which means that oxidative stress and it's damage has been reduced. This study found that blackthorn flower extract has many bioactive compounds, which in combination with using high-protein diet (whey protein) have positive effect in reducing oxidative stress and increasing the activity of endogenous antioxidant enzymes.

• Keywords: ekstrakt cvijeta trnine; lipidna peroksidacija; superoksid dismutaza; reducirani glutation
• Keywords (english): blackthorn flower extract; lipid peroxidation; superoxide dismutase; reduced glutathione
• Language: croatian
• URN:NBN: urn:nbn:hr:159:490655
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 09:10:25