Utjecaj sastava hranjive podloge na rast i aktivnost bakterija mliječne kiseline

undergraduate thesis

• Title (english): Effect of growth medium composition on the growth and activity of lactic acid bacteria
• Author: Ines Ivanuša
• Mentor: Anita Slavica (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2011-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Primjena bakterija mliječne kiseline ima dugu tradiciju u biotehnološkoj industrijskoj proizvodnji fermentiranih proizvoda. Sastav podloga u kojima se ove bakterije uzgajaju i aktivne su podržava specifične zahtjeve ovih kutura za hranjivim tvarima. Takve podloge obično sadrže jedan ili više izvora ugljika, vodika, dušika, fosfora, sumpora kao i određene sastojke koji podržavaju fermentativni metabolizam ovih bakterija. Neke vrste bakterija mliječne kiseline imaju genetički potencijal koji podržava aerobni (respiratorni) metabolizam. U podloge u kojima se u stanicama ovih bakterija uspostavlja respiratorni metabolizam potrebno je dodati izvore hema ili, u nekim slučajevima, menakinona. Tijekom respiratornog metabolizma pridobiva se više metaboličke energije, smanjuje se koncentracija otopljenog kisika i proizvedene mliječne kiseline, dok se koncentracija bakterijske biomase povećava kao i preživljavanje stanica u odabranim uvjetima bioprocesa. Odvijanje aerobnog metabolizma u stanicama bakterija mliječne kiseline ima industrijsku primjenu u proizvodnji starter kultura.

Abstract (english)

Lactic acid bacteria have been traditionally applied in biotechnological industrial production of fermented products. The bacteria are usually cultivated in media which composition supports specific requirements of those cultures for nutrients. Such media usually contain one or more carbon sources as well as sources of hydrogen, nitrogen, phosphorus, sulphur, and specific ingredients which maintain fermentative metabolism of the bacteria. Some species of lactic acid bacteria have genetic potential which supports aerobic (respiration) metabolism. Respiration metabolism can be established in cells in media with exogenous source of heme and, in some cases, menaquinones. During respiration metabolism more metabolic energy can be yielded, concentration of dissolved oxygen and produced lactic acid is reduced while concentration of bacterial biomass and survival of the cells in bioprocess conditions are enhanced. Aerobic metabolism in the cells of lactic acid bacteria has found its industrial implemetation for the production of starter cultures.

• Keywords: sastav hranjive podloge; bakterije mliječne kiseline; fermentativni metabolizam; hem i menakinoni; aerobni metabolizam
• Keywords (english): growth medium composition; lactic acid bacteria; fermentative metabolism; heme and menaquinones; aerobic metabolism
• Language: croatian
• URN:NBN: urn:nbn:hr:159:054969
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-19 09:02:16