Utjecaj pojedinih proteina stanične stijenke kvasca Saccharomyces cerevisiae na poroznost stijenke

undergraduate thesis

• Title (english): Influence of individual cell wall proteins on permeability of the Saccharomyces cerevisiae cell wall
• Author: Ivana Martinić
• Mentor: Renata Teparić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2011-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Stanična stijenka kvasca Saccharomyces cerevisiae je ekstracelularni organel vrlo dinamične strukture koji ima funkciju zaštitnog omotača. Sastavljena je od slojevite mreže β-glukana, hitina i manoproteina. Ovaj organel igra kritičnu ulogu u zadržavanju integriteta stanice tijekom njenog rasta i u uvjetima stresa. Proteini stanične stijenke su raznoliki, a upravo ova raznolikost igra važnu ulogu u prilagodbi stanice na okolišne uvjete te u njenom preživljavanju. Konstrukcija stijenke je kontrolirana fazom staničnog ciklusa pa stijenke stanica u eksponencijalnoj fazi imaju veću permeabilnost od onih u stacionarnoj fazi. U ovom radu ispitana je poroznost stijenke stanica u eksponencijalnoj fazi praćenjem izlučivanja enzima kisele fosfataze u medij. Rezultati pokazuju da se kod suviška Scw4p proteina poroznost stijenke povećava te se kod nedostatka i Scw4p i Scw10p proteina drastično smanjuje. Također je utvrđeno da nedostatak GPI proteina značajno povećava propusnost dok je za PIR proteine pokazano da praktički ne utječu na propusnost stanične stijenke.

Abstract (english)

Cell wall of the yeast Saccharomyces cerevisiae is extracellular organelle with highly dynamic structure which has function of protective envelope. It´s consist of a layered network of β-glucan, chitin and manoproteins. This organelle plays a critical role in maintaining the integrity of the cells during it´s growth and in stress conditions. Proteins of the cell wall are diverse and it´s diversity plays an important role in cell adaptation to environmental conditions and it´s survivor. Construction of the wall is controlled by the phase of the cell cycle so cell wall in the exponential phase have a higher permeability than those in a stationary phase. This thesis discusses the porosity of the wall cells in the exponential phase by monitoring of excretion of the enzyme acid phosphatase into the medium. The results show that excess of the Scw4p protein increases the porosity of the wall and the lack of the Scw4p and Scw10p protein drastically reduce the porosity. Also it was found that the lack of GPI proteins significantly increases the permeability while the lack of PIR proteins practically has no effect on cell wall permeability.

• Keywords: Saccharomyces cerevisiae; stanična stijenka; proteini stanične stijenke; poroznost
• Keywords (english): Saccharomyces cerevisiae; cell wall; cell wall proteins; porosity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:324746
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-19 11:48:49