Kinetika proizvodnje bioetanola s kvascem Saccharomyces cerevisiae na sirovom soku šećerne repe

master's thesis

• Title (english): Kinetics of bioethanol production with yeast Saccharomyces cerevisiae on raw sugar beet juice
• Author: Ivan Rolih
• Mentor: Predrag Horvat (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2011-05-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Istražena je dinamika ekstrakcije saharoze iz rezanaca šećerne repe vodom temperature 20, 28, 36, i 72 °C, za laminarni režim strujanja kroz ekstrakcijsku kolonu. Postavljene su polinomna i eksponencijalna jednadžba za ovisnost koncentracije šećera u ekstraktu o temperaturi i vremenu ekstrakcije. Istražen je inhibicijski učinak visokih koncentracija supstrata na rast kvasca Saccharomyces cerevisiae u anaerobnim uvjetima i eksperimentalno su određeni kinetički parametri za ukupne šećere (KS = 52,411 g/L, μMAX =0,425 h-1, KI = 122,112 g/L) i za glukozu (KS = 9,079 g/L, μMAX =0,301 h-1, KI = 36,084 g/L). Diferencijalnom metodom testiran je inhibicijski učinak koncentracije etanola [0-13 % (vol/vol)] na kinetiku rasta proizvodnog soja i određeni su kinetički parametri za tri formalno kinetička modela. Najbolje slaganje s eksperimentalnim rezultatima postignuto je modelom prema Jerusalimskom (Jerusalimsky, 1968) uz kinetičke parametre (μMAX = 0,430 h-1, KP = 2,269 g/L).

Abstract (english)

The dynamics of sucrose extraction from sugar beet was investigated by using water at different temperatures (20, 28, 36, and 72 ° C) and laminar flow through the extraction column. Polynomial and exponential equations were selected to describe the relation between sugar concentration (at different extraction temperatures) and extraction time. Under anaerobic conditions, inhibitory effect of high different substrate concentrations on the growth rate of yeast Saccharomyces cerevisiae was studied. Experimentally determined kinetic parameters of these cultivations were: KS = 52,411 g/L, μMAX =0,425 h-1, KI = 122,112 g/L; and for glucose: KS = 9,079 g/L, μMAX =0,301 h-1, KI = 36,084 g/L. Furthermore, the inhibitory effect of ethanol concentration (0 - 13 % vol/vol) on anaerobic yeast cultivation was examined by differential method. For this anaerobic yeast cultivation, three formal kinetic growth models were established. On the basis of obtained results, Jerusalimsky model shows the best agreement with experimental results by following kinetic parameter values: μMAX = 0,430 h-1 and KP = 2,269 g/L.

• Keywords: bioetanol; Saccharomyces cerevisiae; inhibicija supstratom; inhibicija produktom; kinetički parametri; sirovi sok šećerne repe
• Keywords (english): bioetanol; Saccharomyces cerevisiae; substrate inhibition; product inhibition; kinetics parameters; raw sugar beet juice
• Language: croatian
• URN:NBN: urn:nbn:hr:159:324090
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-30 08:19:17