Utjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis

master's thesis

• Title (english): Influence of medium composition and environmental conditions on the production of S-adenosyl-L-methionine with the yeast Pichia stipitis
• Author: Ana Percela
• Mentor: Damir Stanzer (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2011-04-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

(S)-adenozil-L-metionin (SAMe) je molekula važna za život svake stanice, jer je univerzalni donor metilne skupine. Nastaje sintezom iz ATP-a i metionina djelovanjem metionin-adenoziltransferaze. Sudjeluje u tri važna metabolička puta: transmetilacija, trans-sulfuracija te biosinteza poliamina. Nedostatak SAMe u ljudskom organizmu kao i poremećaji u metabolizmu SAMe mogu se manifestirati kroz različite poremećaje u funkcioniranju jetre, te imunološkom i živčanom sustavu. Cilj ovog rada bio je proučiti utjecaj sastava podloge i uvjeta okoline na proizvodnju SAMe pomoću kvasca Pichia stipitis pri uzgoju na laboratorijskoj tresilici. Rezultati pokazuju da se najveća količina akumuliranog SAMe ostvaruje u 18-om satu uzgoja (69,63 mg/g s.tv.kvasca) u glukoznoj podlozi s dodatkom 4,5 g/L D,L-metionina, pri temperaturi od 30oC i pH vrijednosti 4,8.

Abstract (english)

S-Adenosyl-L-methionine (SAMe) is important for all living cells, because it is universal methyl donor. This substance is synthesized from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase. SAMe participates in three important metabolic pathways: transmethylation, transsulfuration and the polyamine biosynthesis. Deficiency of SAMe in human organism as well as abnormalities in SAMe metabolism can be manifested through different disfunctions in functioning of a liver, immunological and nervous system. The aim of this work was determine the influence of medium composition and environmental conditions on the production of SAMe in the yeast Pichia stipitis, grown at laboratory shaker. The results showed that Pichia stipitis reaches maximal SAMe accumulation in 18th hour of cultivation (69.63 mg/g dry cells Yeast) in glucose media with the addition of 4.5 g/L D,L-methionine, at a temperature of 30° C and pH value 4.8.

• Keywords: kvaščeva biomasa; S-adenozil-L-metionin
• Keywords (english): S-Adenosyl-L-methionine; yeast biomass
• Language: croatian
• URN:NBN: urn:nbn:hr:159:873851
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-30 10:43:50