Uspoređivanje klasičnih mikrobioloških i biokemijskih metoda u određivanju mikrobne populacije iz namirnica životinjskog podrijetla

master's thesis

• Title (english): Comparison of conventional microbiological and biochemical methods in the determination of microbial populations of food of animal origin
• Author: Doris Alajbegović
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2011-02-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Konvencionalne mikrobiološke metode za detekciju patogena su dugotrajne, ali iako postoje mnoge brže metode, još uvijek ostaju službena korištena metoda. Svrha ovog rada bila je usporediti mikrobiološke i biokemijske metode u određivanju patogenih mikroorganizama iz uzoraka domaćih kobasica, slavonskog kulena, svježeg kravljeg sira i vrhnja od sirovog mlijeka. Od ukupno 45 analiziranih uzoraka namirnica životinjskog podrijetla, 24,4 % uzoraka su bili pozitivni na Listeria vrste na PALCAM agaru, a u samo 8,88 % uzoraka API- Listeria testom je potvrđena L. monocytogenes. Od 37,7 % uzoraka pozitivnih na stafilokoke na Baird-Parker agaru, API Staph testom u 6,66 % uzoraka potvrđena je Staphylococcus aureus. Bakterija iz roda Salmonella sp. dokazana je API 20 E testom u samo jednom uzorku domaće kobasice.

Abstract (english)

Conventional microbiological methods for detection of pathogens are time consuming, but still remain the official method used despite there are many faster methods. The purpose of this study was to compare the microbiological and biochemical methods to determine the pathogenic microorganisms from samples of domestic sausages, Slavonian kulen, fresh cheese and cream of raw milk. From a total of 45 tested samples, 24,4 % were positive for Listeria on PALCAM agar and only 8,8 % samples were confirmed to be positive for L. monocytogenes by API-Listeria test. Of 37.7% of samples that were positive for staphylococci on Baird-Parker agar, the API Staph test confirmed Staphylococcus aureus in 6,66 % of samples. API 20 E test confirmed the bacteria Salmonella sp. in only one sample of domestic sausage.

• Keywords: namirnice životinjskog podrijetla; patogeni mikroorganizmi; klasične mikrobiološke metode; API test
• Keywords (english): foods of animal origin; pathogens microorganisms; conventional microbiological methods; API test
• Language: croatian
• URN:NBN: urn:nbn:hr:159:018737
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-30 11:24:19