Analitičke metode u procesima biosorpcije

master's thesis

• Title (english): Analytical methods in biosorption process
• Author: Barbara Vrdoljak
• Mentor: Jasna Mrvčić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2011-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Istražene su analitičke metode za praćenje učinkovitosti vezanja esencijalnih iona metala na odabrane vrste bakterija mliječne kiseline. Istražene su metode elektronske mikroskopije, FT-IR spektroskopije te Langmirov-matematički model. Rezultati su pokazali da se elektronska mikroskopija može primijeniti za određivanje mjesta vezanja iona metala s određenim ograničenjima, jer neki metali, kao što je kadmij i bakar, u transmisijskoj elektronskoj mikroskopiji nisu vidljivi. FT -IR spektroskopija je pouzdana, brza, jednostavna i jeftina metoda za definiranje mehanizma vezanja iona metala s pomoću mikrobne biomase. Rezultati procesa biosorpcije za sve ispitivane vrste bakterija slijede Langmuir-ov model, što je izraženo visokim R2 vrijednostima.

Abstract (english)

The analytical methods: electron microscopy, FT-IR spectroscopy and Langmuir mathematical model, for the efficiency of essential metal ions binding on selected species of lactic acid bacteria was investigated. The results showed that electron microscopy can be used to determine metal ions binding by LAB with certain restrictions, for some metals such as cadmium and copper, which in transmission electron microscopy are not visible. FT-IR spectroscopy is a reliable, fast, simple and inexpensive method for defining the mechanism of metal ions binding by microbial biomass. The correlation regression coefficients show that the biosorption process can be well defined by the Langmuir equation.

• Keywords: bakterije mliječne kiseline; biosorpcija; biogeni elementi
• Keywords (english): lactic acid bacteria; biosorption; trace elements
• Language: croatian
• URN:NBN: urn:nbn:hr:159:492566
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-31 09:47:49