Fenotipska karakterizacija bakterija mliječne kiseline iz hrvatskih autuhtonih prehrambenih proizvoda usporedbom klasične metode i metoda spektrometrije masa

master's thesis

• Title (english): Comparison of classical method and mass spectrometry methods for phenotypic characterization of lactic acid bacteria from croatian authentic food
• Author: Marina Grandić
• Mentor: Višnja Bačun-Družina (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2011-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Danas postoje sve veći zahtjevi za brzim metodama u identifikaciji i karakterizaciji mikrobnih vrsta. Cilj ovog istraživanja bio je fenotipski karakterizirati bakterije mliječne kiseline iz šesnaest autohtonih hrvatskih proizvoda. Različite tehnike spektrometrije masa zbog brzine izvedbe i pouzdanosti imaju potencijal da zamijene standardne fenotipske i genotipske metode koje se rutinski rabe za identifikaciju bakterijskih vrsta. Usporedbom dvije MS metode za identifikacija vrsta, zaključeno je kako je hibridna tehnika iščitavanja de novo točnija od metode MALDI-TOF Biotyper. U ovom radu ispitano je bakteriocidno djelovanje izoliranih bakterija mliječne kiseline iz autohtonih hrvatskih proizvoda te je utvrđen povećani bakteriocidni potencijal u bakterija Lactobacillus plantarum, Lactobacillus casei i Pediococcus pentosaceus. Utvrđena je različita antibiotička osjetljivost u deset sojeva L. plantarum na cefalosporine treće generacije.

Abstract (english)

Nowdays there are increasing demands for rapid methods in the identification and characterization of microbial species. The aim of this study was to phenotypically characterize lactic acid bacteria from sixteen authentic Croatian products. Different techniques of mass spectrometry due to the speed of performance and reliability have the potential to replace standard phenotypic and genotypic methods that are routinely used for identification of bacterial species. Comparing the two MS methods for species identification led to the conclusion that the hybrid techniques de novo sequencing is a more precise method than MALDI-TOF Biotyper. In this study the bacteriocidal effect of lactic acid bacteria has been isolated and an increased bacteriocidal potential of Lactobacillus plantarum, Lactobacillus casei and Pediococcus pentosaceus has been identified. The results showed a different antibiotic sensitivity of nine strains of L. plantarum to the third-generation cephalosporins.

• Keywords: bakterije mliječne kiseline; identifikacija vrsta; spektrometrija masa; antibiotička rezistencija
• Keywords (english): lactic acid bacteria; species identification; mass spectrometry; antibiotic resistance
• Language: croatian
• URN:NBN: urn:nbn:hr:159:156329
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-31 10:07:40