Mikrobiološka ispravnost narezaka trajnih kobasica pakiranih u modificiranoj atmosferiMicrobiological safety of sliced shelf stable sausages packed in modified atmosphere

master's thesis

• Title (english): Microbiological safety of sliced shelf stable sausages packed in modified atmosphere
• Author: Mateja Mirkov
• Mentor: Frane Delaš (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2011-12-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Svrha ovog diplomskog rada bila je odrediti moguću prisutnost bakterije Listeria monocytogenes u istraživanim uzorcima narezaka trajnih kobasica pakiranih u modificiranoj atmosferi, te u skladu s dobivenim rezultatima utvrditi udovoljavaju li istraživani uzorci mikrobiološkim standardima za namirnice sukladno Pravilniku o mikrobiološkim kriterijima za hranu (NN 74/08). Posebna pažnja posvećena je dokazivanju moguće prisutnosti bakterije Listeria monocytogenes u navedenim uzorcima zbog njezine izrazite opasnosti po zdravlje u ljudi. Metode za dokazivanje ove bakterije uključuju primarno obogaćivanje u 1/2 Fraser bujonu, slijedi selektivno obogaćivanje u Fraser bujonu, te nacjepljivanje na krutu selektivnu podlogu, ALOA agar. Daljnja identifikacija uključuje katalaza test (katalaza pozitivna), bojanje po Gramu (gram - pozitivni štapići), test pokretljivost (pokretna), test hemolize (beta hemoliza) i iskorištavanje ugljikohidrata (iskorištava ramnozu, ali ne i ksilozu). Od 60 nasumično odabranih narezaka trajnih kobasica pakiranih u modificiranoj atmosferi koji su istraživani na prisutnost bakterije Listeria monocytogenes, svi su potvrđeni kao zdravstveno i mikrobiološki ispravni.

Abstract (english)

The purpose of this study was to determinate possible presence of bacteria Listeria monocytogenes in analysed sliced shelf stable sausages packed in modified atmosphere and to determine whether the samples meet microbiological requirements according to the Regulation on Microbiological Criteria for Foods (OG 74/08). Particular attention is given to investigate bacteria Listeria monocytogenes possible presence in these samples, because of its exceptional hazard to human health. Methods for identification of this bacteria include: pre - enrichment in Half - Fraser broth, selective enrichment in Fraser broth, inoculation on the surface of solid selective medium Aloa agar. Further identification includes catalase test (catalase positive), colouring per Gram (gram – positive rods), mobility test (movable), haemolysis test (beta haemolysis), carbohydrate use (uses rahmnose, but not xylose). 60 randomly chosen samples of sliced shelf stable sausages packed in modified atmosphere have been analysed on presence of bacteria Listeria monocytogenes. All analysed samples were confirmed as microbiology safe, as no Listeria monocytogenes was identify.

• Keywords: izolacija; Listeria monocytogenes; mikrobiološka ispravnost; modificirana atmosfera; trajne kobasice.
• Keywords (english): isolation; Listeria monocytogenes; microbiological safety; modified atmosphere; shelf stable sausages.
• Language: croatian
• URN:NBN: urn:nbn:hr:159:363503
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-31 10:25:12