Utjecaj MnSO4×H2O na kinetiku alkoholne fermentacije i ugradnju mangana u biomasu kvasca Saccharomyces cerevisiae

master's thesis

• Title (english): The impact of MnSO4×H2O on kinetics of alcohol fermentation and accumulation of manganese in biomass of yeast Saccharomyces cerevisiae
• Author: Nikolina Kukić
• Mentor: Damir Stanzer (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2011-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Istraživan je utjecaj različitih koncentracija manganova sulfata u melasnoj i saharoznoj podlozi na proizvodnju etanola i prirast kvaščeve biomase tijekom 24 sata. Fermentacije su provedene u dinamičkim uvjetima (na tresilici), sa i bez dodatka manganova sulfata u podlogu. Ioni mangana u količini koja odgovara do 30 mg/L dodanog manganova sulfata pokazali su pozitivan utjecaj na kinetiku alkoholne fermentacije na saharoznoj podlozi u dinamičkim uvjetima, dok su veće koncentracije navedene soli djelovale inhibicijski. Koncentracije manganova sulfata do 300 mg/L u melasnoj podlozi nisu uzrokovale znatne promjene u kinetici alkoholne fermentacije. Također je utvrđeno da brzina akumulacije iona mangana u biomasu ovisi o soju kvasca i aerobnosti procesa (soj SM2 akumulira više iona mangana u dinamičkim i aerobnim uvjetima, a soj TVG4 u statičkim). Najveća brzina akumulacije primjećena je u početnim fazama staničnog rasta. Akumulirana količina mangana je bila veća u statičkim (3,67 mg/g s. tv.) i dinamičkim (2,11 mg/g s. tv.) uvjetima, nego u aerobnim uvjetima (1,46 mg/g s. tv.).

Abstract (english)

The impact of different manganese sulphate concentrations on kinetics of yeast biomass growth and the ethanol production was determined in the molasses and sucrose medium during 24 hours. Fermentations were performed in dynamic conditions (on rotating shaker), with and without addition of manganese sulphate in the medium. Up to 30 mg/L of manganese sulphate had positive effect on alcoholic fermentation in sucrose media at dynamic conditions, while higher concentrations had the inhibitory effect. The amounts of manganese sulphate up to 300 mg/L in the molasses media did not cause any substantial changes in kinetics of alcoholic fermentation. It was determined that the rate of manganese ions accumulation depends on yeast strain, the phase of yeast growth and the rate of the oxygen supply. Yeast strain SM2 accumulates more manganese ions in dynamic (2.11 mg/g d.m.) and aerobic (1.46 mg/g d.m.) conditions, while strain TVG4 accumulates more manganese ions in static condition (3.67 mg/g d.m.).

• Keywords: kvasac Saccharomyces cerevisiae; alkoholna fermentacija; mangan; mikroelementi
• Keywords (english): yeast Saccharomyces cerevisiae; alcoholic fermentation; manganese; microelements
• Language: croatian
• URN:NBN: urn:nbn:hr:159:845827
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-31 10:37:16