The aim of this work was to investigate adhesion ability of bacterial strain Lactococcus lactis ssp. lactis 5K1, isolated from "horse meat saussages", on the small and large intestine of mice in vitro and in vivo conditions also his ability to survive conditions in the intestinal tract. Considering that in preliminary investigation L. plantarum 1K has shown good antimicrobial activity against pathogen microorganisma from food, has produced significant amount of lactic acid (17,23 g/L)and satisfied the basic criteria for selection of probiotic strains in vitro conditions, in vivo studies in experimental mice were carried out. Lactococcus lactis ssp. lactis 5K1 survive the conditions in the intestinal tract and significantly reduced the number of bacterial cells of Salmonella sp. in mice infected with bacterium Salmonella sp. Considering that Lactococcus lactis ssp. lactis 5K1 has demonstrated the basic functional criteria for the selection of probiotic strains, as such, it can be used as functional autochthonous starter culture for fermented meat products.