Utjecaj vrste otapala i temperature na ekstrakciju polifenola kadulje (Salvia officinalis L.)

undergraduate thesis

• Title (english): Effect of solvent and temperature on the extraction of polyphenols sage (Salvia officinalis L.)
• Author: Jelena Dragosavljević
• Mentor: Verica Dragović Uzelac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2010-07-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Istraživan je utjecaj otapala i temperature na ekstrakciju fenolnih spojeva samonikle kadulje (Salvia officinalis L). Za provođenje ekstrakcije korištena su vodene otopine etanola i acetona različitih koncentracija (30%, 50%, 70%-tna vodena otopina etanola i acetona) te tri različite temperature ekstrakcije (30, 50, 70°C). U ekstraktima je provedeno određivanje ukupnih fenola i flavonoida spektrofotometrijski. Najviša koncentracija ukupnih fenola (42,05 i 42,30 mg RAE/g suhe kadulje) određena je u ekstraktima dobivenim upotrebom 70%-tnog etanola pri 70°C i 50%-tnog acetona pri 50°C. Kako je vrelište acetona nisko i on ekstrahira i neke druge spojeve koji apsorbiraju pri 760nm, utvrđeno je da je etanol bolje otapalo za ekstrakciju. Koncentracije ukupnih fenolnih spojeva u ekstraktima dobivenim upotrebom 50%-tnog i 70%-tnog etanola pri 70°C bile su približne te je 50%-tna vodena otopina etanola izabrana kao najučinkovitije otapalo za ekstrakciju fenolnih spojeva iz kadulje zbog manjeg udjela etanola.

Abstract (english)

The influence of solvent and temperature on the extraction of phenolic compounds of dry wild sage (Salvia officinalis L) was studied. Extraction was carried out using aqueous solutions of ethanol and acetone of different concentrations (30%, 50%, 70%) as solvents at three different extraction temperatures (30, 50, 70°C). Total phenolics and flavonoids were spectrophotometrically determined in all prepared extracts. The highest concentration of total phenolics (42.05 and 42.30 mg RAE / g dry sage) was determined in extracts obtained using 70% ethanol at 70°C and 50% acetone at 50°C. Because of the low boiling point and lower selectivity of acetone, it was found that ethanol is a better solvent for extraction of phenols. The concentrations of total phenolic compounds in the extracts obtained using 50% and 70% ethanol at 70°C were approximately the same, so the 50% aqueous solution of ethanol was chosen as the most effective solvent for extraction of phenolic compounds from sage due to a lower share of ethanol.

• Keywords: kadulja; fenolni spojevi; flavonoidi; etanol; aceton
• Keywords (english): sage; phenolic compounds; flavonoids; ethanol; acetone
• Language: croatian
• URN:NBN: urn:nbn:hr:159:272407
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-06-02 10:19:32