Priprema i karakterizacija novih piridinijevih soli - mogućih obilježivača nukleinskih kiselina

undergraduate thesis

• Title (english): Preparation and characterization pirydinium salts possible markers of nucleic acid
• Author: Vanja Ivanković
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2010-09-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cijaninske boje (stirilpiridinijeve soli) tip su vrlo važnih funkcionalnih boja koje se široko primjenjuju u mnogim područjima tehnologije kao fluorescentni obilježivači za stanice, micele i organele. U ovom je radu cilj bio sintetizirati dvije nove soli derivata piridoksina: 3- hidroksi-4,5-bis(hidroksimetil)-1-metil-2-(4-N,N-diaminostiril)piridinijev jodid[1] i 3- hidroksi-4,5-bis(hidroksimetil)-1-metil-2-[(4-metoksifenil)-3-metil-1,3-dien-1-il]piridinijev jodid [2]. Ovi spojevi su pripravljeni reakcijom 3-hidroksi-4,5-bis(hidroksimetil)-1,2- dimetilpiridinijeva jodida s 4-N,N-dimetilaminobenzaldehidom te 3-(4-metoksifenil)-2- metilprop-2-enalom. Struktura novih, do sada neobjavljenih, spojeva [1] i [2] odreñena je pomoću podataka dobivenih IR-spektroskopijom te 1H- i 13C- nuklearno-magnetskom rezonancijom.

Abstract (english)

Cyanine dyes (styrylpyridinum salts) are a type of a very important functional dyes that are widely applied in many areas of technology such as flourescent labels for cells, micelles and organelles. In this final work, the ultimate goal was the synthesis of two new pyridoxine salts: 3-hydroxy-4,5-bis(hydroxymethyl)-1-methyl-2-(4-N,N-diaminostyryl) pyridinium iodide [1] and 3-hydroxy-4,5-bis(hydroxymethyl)-1-methyl-2-[(4- methoxyphenyl)-3-methyl-1,3-dien-1-yl]pyridinium iodide [2]. These compounds are prepared by reaction of 3-hydroxy-4,5-bis(hydroxymethyl)-1,2-dimethylpyridinium iodide with 4-N,N-dimethylaminobenzaldehyde and 3-(4-methoxyphenyl)-2-methylprop-2-enal. Structures of new, yet unpublished compounds [1] and [2] were determined according to the data obtained by IR-spectroscopy as well as 1H- and 13 C-nuclear magnetic resonance.

• Keywords: kvarterne soli; N-metilpiridoksin; obilježivači DNA; stirilpiridinijeve soli
• Keywords (english): quarternary salts; N-methylpyridoxine; labels of DNA; styrylpyridine salts
• Language: croatian
• URN:NBN: urn:nbn:hr:159:023231
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-06-07 11:35:36