The effects of aflatoxin B1 and ochratoxin A on the morphological characteristics of lactic acid bacteria

undergraduate thesis

• Title: Utjecaj aflatoksina B1 i okratoksina A na morfološke osobine bakterija mliječne kiseline
• Author: Romano Šunjić
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2016-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj rada je istražiti utjecaj aflatoksina B1 (AFB1) i okratoksina A (OTA) na preživljavanje i morfološke osobine bakterije Lactobacillus plantarum 1K. Toksično djelovanje mikotoksina, osim na čovjeka, životinje i biljke, dokazano je i u mikroba (bakterije, kvasci, plijesni, protozoe), a najčešće kroz inhibiciju rasta. Mikrobni rast ovisi o uvjetima okoline u kojoj se mikrobi nalaze kao i izloženosti kemijskim agensima, a prikazuje se najčešće u obliku krivulje rasta. Krivulja rasta bakterije Lactobacillus plantarum 1K određivana je u prisutnosti AFB1 i OTA tijekom 48 sati uzgoja metodom neizravnog određivanja broja živih stanica, brojanjem poraslih kolonija na čvrstoj hranjivoj podlozi izraženih kao log10 CFU/mL. Morfološke osobine stanica, kao što su dužina i širina, određene su metodom mikrometrije upotrebom objektnog i okularnog mikrometra. Iz dobivenih rezultata uočeno je da oba istraživana mikotoksina utječu i na bakterijski rast i na dimenzije stanica bakterije L. plantarum 1K u ovisnosti o vremenu.

Abstract (english)

The aim of this study was to investigate the impact of aflatoxin B1 (AFB1) and ochratoxin A (OTA) on survival and morphological characteristics of Lactobacillus plantarum 1K. Toxic effects of mycotoxins, in addition to man, animals and plants have been proven on microbes (bacteria, yeasts, molds, protozoa), but mostly trough the inhibition of growth. Microbial growth is dependent on the environmental conditions in which the microbes are as well as exposure to chemical agents, and most often is shown in form of growth curves. The growth curve of Lactobacillus plantarum 1K was determined in the presence of AFB1 and OTA during 48 hours of cultivation by method of indirect determination of the number of living cells, by counting the colonies grown on the solid culture medium, expressed as log10 CFU/mL. Morphological characteristics of cells, such as length and width, were determined by using micrometry object and ocular micrometer. From the results it was observed that both mycotoxins affect the growth of bacterial cells and the dimensions of L. plantarum 1K versus time.

• Keywords: aflatoksin B1; okratoksin A; Lactobacillus plantarum; krivulja rasta; morfološke osobine bakterija
• Keywords (english): aflatoxin B1; ochratoxin A; Lactobacillus plantarum; growth curve; morphological characteristics of bacteria
• Language: croatian
• URN:NBN: urn:nbn:hr:159:970544
• Study programme: Title: Biotehnologija; Study programme type: sveučilišni; Study level: preddiplomski; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 11:47:24