Uzgoj alge Euglena gracilis s ciljem proizvodnje vitamina A i vitamina E

master's thesis

• Title (english): Cultivation of algae Euglena gracilis for vitamin A and vitamin E production
• Author: Marina Bujić
• Mentor: Tonči Rezić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2010-03-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom istraživanju proveden je uzgoj alge Euglena gracilis s ciljem proizvodnje vitamina A i vitamina E. Proces optimizacije sastava hranjive podloge i uvjeta uzgoja E. gracilis, u Erlenmeyer tikvicama na laboratorijskoj tresilici, proveden je u svrhu određivanja najpovoljnijih parametara za proizvodnju vitamina u bioreaktoru. Tijekom uzgoja alge E. gracilis u bioreaktoru s mješalom primijenjeni su različiti načini uzgoja (šaržni i šaržni s pritokom supstrata) da bi se odabrao najpovoljniji način za industrijsku primjenu u proizvodnji vitamina. Veće produktivnosti procesa proizvodnje vitamina A i vitamina E zabilježene su kod fotomiksotrofnog, šaržnog uzgoja s pritokom supstrata na kemijski definiranoj podlozi.

Abstract (english)

In this research, cultivation of algae Euglena gracilis was studied in order to produce vitamin A and vitamin E. The optimization of medium composition and cultivation conditions, in Erlenmeyer flasks on laboratory shaker, was done to define the most appropriate parameters for vitamin production in stirred tank bioreactor. During cultivation of E. gracilis in stirred tank bioreactor different cultivation techniques (batch and fed batch) were examined in order to define the most appropriate technique for industrial use. In fed batch cultivation, where cells were grown photomixotrophically on chemical defined medium, efficiency of bioprocess were bigger then in batch cultivation of E. gracilis on composite medium.

• Keywords: Euglena gracilis; vitamin A; vitamin E; fotomiksotrofni uzgoj; sastav hranjive podloge
• Keywords (english): Euglena gracilis; vitamin A; vitamin E; photomixotrophic cultivation; broth composition
• Language: croatian
• URN:NBN: urn:nbn:hr:159:948149
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-25 12:01:39