Glukozinolati i polifenolni spojevi u različitim sortama brokule

master's thesis

• Title (english): Glucosinolates and polyphenol compounds in different broccoli cultivars
• Author: Željka Mirenić
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2010-06-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Brokula je dobar izvor za zdravlje važnih nutrijenata poput glukozinolata i polifenola. U ovom je radu provedena analiza pojedinačnih i ukupnih glukozinolata, ukupnih polifenolnih spojeva i njihova antioksidacijskog kapaciteta, u trinaest različitih sorti brokule. Glukozinolati (GSL) su kvantificirani tekućinskom kromatografijom obrnutih faza (RP-LC DSGSL) međunarodnom standardnom metodom (ISO 10633-1, 1995) koja uključuje ekstrakciju metanolom, enzimsku desulfataciju i tekućinsku kromatografiju obrnutih faza (RP-LC) . Identifikacija desulfoglukozinolata provedena je prema redoslijedu eluiranja s kromatografske kolone i analizom UV spektara (Diode Array detekcija). Udio ukupnih glukozinolata u istraživanim sortama brokule jest između 5,14 i 14,92 µmol g-1 uzorka. Ukupni polifenoli analizirani su UV/Vis spektroskopskom metodom uporabom Folin-Ciocalteau (FC) reagensa. Udio polifenolnih spojeva u istraživanim sortama jest u rasponu od 8,21 do 13,98 mg g-1 s.t.. Određivanje antioksidacijskog kapaciteta provedeno je spekrofluorimetrijskom ORAC metodom. Onaj uzorak brokule koji ima veću koncentraciju polifenola pokazuje i veći antioksidacijski kapacitet.

Abstract (english)

Broccoli is a good source of health promoting compounds such as glucosinolates and polyphenols. In this study we analyzed total and individual glucosinolates, total polyphenols and antioxidative capacity in thirteen different broccoli cultivars. Glucosinolates (GSL, were quantified by Standard method for determination of glucosinolates (ISO 10633-1, 1995) and it includes extraction with methanol, enzymatic desulphation and Reversed-Phase Liquid Chromatography (RP-LC). The identification of desulphoglucosinolates eluted was made according to the elution order from chromatographic column and also by UV-spectar analyses (Diode Array detection). The content of total glucosinolates in the cultivars investigated ranged between 5.14 and 14.92 µmol g-1 sample. Total polyphenols were determinated by UV/Vis spectroscopic method using Folin-Ciocalteau reagent. Polyphenol quantity in the cultivars investigated ranged between 8.21 and 13.98 mg g-1 d.w.. Antioxidative capacity was measured by the Oxygen Radical Absorbance Capacity (ORAC) method. Antioxidative activity of broccoli was higher in samples that have higher total amount of polyphenols.

• Keywords: brokula; glukozinolati; ORAC; polifenoli; RP-LC
• Keywords (english): broccoli; glucosinolates; ORAC; polyphenols; RP-LC
• Language: croatian
• URN:NBN: urn:nbn:hr:159:084391
• Study programme: Title: Food Technology; specializations in: Nutrition; Course: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer prehrambene tehnologije; smjer nutricionizam (diplomirani inženjer prehrambene tehnologije; smjer nutricionizam)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-29 07:41:08