Određivanje kafeina u osvježavajućim bezalkoholnim pićima tekućinskom kromatografijom visoke djelotvornosti

master's thesis

• Title (english): Determination of caffeine in non-alcoholic beverages by high performance liquid chromatography
• Author: Marijo Leško
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2010-12-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kafein (1,3,7-trimetilksantin) je alkaloid koji se dodaje u osvježavajuće bezalkoholne napitke kao psihostimulans radi pojačanja budnosti i pažnje, odnosno skraćivanja vremena reakcije. Cilj rada bio je utvrditi količinu kafeina u devet uzoraka osvježavajućih bezalkoholnih pića primjenom tekućinske kromatografije visoke djelotvornosti (HPLC) uz UV detekciju. Dobiveni rezultati su uspoređeni s propisanim vrijednostima u Pravilniku o temeljnim zahtjevima za ovježavajuća bezalkoholna pića i soda-vodu (NN 23/97) i Pravilniku o hrani za posebne prehrambene potrebe (NN 81/04). Istraživanje je pokazalo da tri analizirana uzorka sadrže količinu kafeina veću od dozvoljene navedenim pravilnicima, dok ostali zadovoljavaju pravilnike.

Abstract (english)

Caffeine (1,3,7-trimethylxanthine) is an alkaloid which is added in a refreshing nonalcoholic beverages as a psychostimulant to boost alertness and attention, and shortening response times. The aim was to determine the amount of caffeine in nine samples of refreshing beverages by using high performance liquid chromatography (HPLC) with UV detection. The results obtained are compared with the values prescribed in the Regulations on the refreshing soft drinks and soda water (NN 23/97) and Regulations on foods for special dietary needs (NN 81/04). Research has shown that the three samples analyzed contain the amount of caffeine greater than allowed by these regulations, while others meet them.

• Keywords: HPLC; kafein; metilksantini; osvježavajuća bezalkoholna pića
• Keywords (english): caffeine; HPLC; methylxanthine; refreshing beverages
• Language: croatian
• URN:NBN: urn:nbn:hr:159:994938
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-29 09:39:23