Heterotrofni uzgoj alge Euglena gracilis s ciljem proizvodnje β-1,3-glukana

master's thesis

• Title (english): Heterotrophic cultivation of algae Euglena gracilis for β-1,3-glucan production
• Author: Marko Dalip
• Mentor: Tonči Rezić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2010-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Glavni cilj ovog istraživanja bio je utvrđivanje najpovoljnijeg načina proizvodnje β-1,3-glukana (paramylona) heterotrofnim uzgojem alge Euglena gracilis u bioreaktoru s mješalom. Paramylon je biološki aktivan polisaharid koji posjeduje imunostimulirajući i antitumorski efekt, snižava razinu kolesterola u krvi, a njegovi sulfidni derivati smanjuju aktivnost HIV-virusa. U ovom istraživanju alga E. gracilis je uzgajana na sintetičkoj hranjivoj podlozi (Hutner), sa različitim izvorima ugljika (glukoza, etanol, Na-acetat) na laboratorijskoj tresilici u Erlenmayer tikvicama i kompleksim podlogama u obliku sekundarnih sirovina (melasa i kukuruzna močevina) u bioreaktoru s mješalom. Uzgoj u bioreaktoru s mješalom proveden je različitim tehnikama uzgoja (šaržno i kontinuirano).

Abstract (english)

The main goal of this research was to define the most favorable cultivation procedure for production of β-1,3-glucan (paramylon) by heterotrophic cultivation of algae Euglena gracilis in stirred tank bioreactor. Paramylon is biologicaly active polisaccharide possesing immunostimulating and an antitumor effect, lowers the blood cholesterol level and his sulphur derivates inhibit the HIV-virus activity. In this investigation algae E. gracilis was cultivated on synthetic (Hutner) medium with different carbon sources (glucose, ethanol, Na-acetate) on shakers in Erlenmayer flasks as well as on complex medium with different industrial by-products (e.g. molasses and corn steep liquor) in stirred tank bioreactor. Cultivation in stirred tank bioreactor was carried out by using different cultivation techniques such as batch and continous cultivation.

• Keywords: Euglena gracilis; β-1; 3-glukan (paramylon); heterotrofni uzgoj; sastav hranjive podloge; bioreaktor s mješalom; različite tehnike uzgoja
• Keywords (english): Euglena gracilis; β-1; 3-glucan (paramylon); heterotrophic cultivation; broth composition; stirred tank bioreactor; different cultivation techniques
• Language: croatian
• URN:NBN: urn:nbn:hr:159:263826
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-29 10:17:16