Izolacija flavonol glikozida iz cvijeta trnine primjenom ekstrakcije potpomognute ultrazvukom

master's thesis

• Title (english): Isolation of flavonol glycosides from blackthorn flowers using ultrasound assisted extraction
• Author: Kristina Čunčić
• Mentor: Verica Dragović Uzelac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2016-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj ovog istraživanja bio je istražiti utjecaj ekstrakcije potpomognute ultrazvukom na prinos flavonol glikozida iz cvijeta trnine, pri čemu su varirani polarnost otapala (50 i 70 % vodena otopina etanola), amplituda ultrazvuka (50, 75 i 100%) i vrijeme ekstrakcije (3, 6 i 9 minuta). Ekstrakcija je provedena pri konstantnoj jakosti ultrazvuka (400W), promjeru sonde (7 mm) i ciklusu (1). Identifikacija i kvantifikacija flavonol glikozida provedena je primjenom ultra djelotvorne tekućinske kromatografije sa masenom spektrometrijom (UPLC-MS/MS). Identificirano je 16 flavonol glikozida i to 8 kamferol glikozida i 7 kvercetin glikozida te izoramnetin-3-rutinozid. Najbolji prinosi flavonol glikozida postižu se uz primjenu 70%-tne vodene otopine etanola, vremenu ekstrakcije 6 minuta i pri amplitudi 50%.

Abstract (english)

The aim of this study was to examine the influence of ultrasound assisted extraction on the yield of flavonol glycosides in blackthorn flowers. The polarity of the solvent (50 and 70% aquaeous ethanol), ultrasound amplitude (50, 75 and 100 %) and extraction time (3, 6, 9 minutes) were varied, whereas intensity of ultrasound (400 W), the diameter of the probe (7 mm) and cycle (1) were constant. Ultra performance liquid chromatography mass spectrometry (UPLC-MS/MS) was used for the identification and quantification of flavonol glycosides. Sixteen flavonol glycosides were identified: 7 quercetin glycosides, 8 kampferol glycosides and isoramnetin-3-rutionoside. The highest flavonol glycosides yield was obtained using 70 % aquaeous solution of ethanol, extraction time 6 minutes and amplitude 50 %.

• Keywords: flavonol glikozidi; ekstrakcija potpomognuta ultrazvukom; trnina ( Prunus spinosa L.); UPLC-MS/MS
• Keywords (english): flavonol glycosides; ultrasound assisted extraction; blackhthorn ( Prunus spinosa L.); UPLC-MS/MS
• Language: croatian
• URN:NBN: urn:nbn:hr:159:071474
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 12:26:27