Titanatne nanocjevčice - novi materijal za kovalentnu imobilizaciju enzima

master's thesis

• Title (english): Titanate nanotubes - new material for covalent immobilization of enzymes
• Author: Vito Teršić
• Mentor: Damir Iveković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Process Engineering); (Laboratory for Measurement, Regulation and Automatisation); Zagreb
• Defense date and country: 2009-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom je radu po prvi puta istražena mogućnost korištenja titanatnih nanocjevčica kao supstrata za imobilizaciju enzima. Titanatne nanocjevčice sintetizirane su hidrotermalnim postupkom, reakcijom između TiO2 i NaOH. Kemijska modifikacija površine nanocjevčica provedena je reakcijom površinskih OH-skupina s (3-aminopropil)trietoksi silanom te glutaraldehidom. Na tako modificirane nanocjevčice kovalentno je vezana katalaza, odabrana kao modelni enzim. Morfološka svojstva nemodificiranih titanatnih nanocjevčica i nanocjevčica s imobiliziranom katalazom određena su transmisijskom i pretražnom elektronskom mikroskopijom, a ionsko-izmjenjivačka svojstva potenciometrijskim titracijama u vodenom mediju. Kemijske modifikacije površine nanocjevčica praćene su FTIR spektroskopijom. Uspješnost kovalentne imobilizacije enzima provjerena je usporedbom katalitičke aktivnosti imobilizirane katalaze s katalitičkom aktivnošću slobodne katalaze u otopini. Dobiveni rezultati pokazali su da je katalazu moguće uspješno imobilizirati na titanatne nanocjevčice, te da tako imobilizirana katalaza pokazuje visoku stabilnost i mogućnost višekratne uporabe enzima, uz minimalan pad katalitičke aktivnosti.

Abstract (english)

In this graduate work for the first time we tried to discover usage of titanate nanotubes for immobilization of enzymes. Titanate nanotubes were synthesized with hydrothermal threatment, by reaction between TiO2 and NaOH. Chemical modification of nanotube surface was made by reaction with surface OH-groups with (3-aminopropyl)trietoksy silane and with glutaraldehyd. On modificated nanotubes we covalently binded catalaze, which is chosen for model enzyme in this work.
Morphological properties non-modificated nanotubes and nanotubes with
immobilizated catalase was determinated with transmission and scanning electronical microscopy, and ion-exchangable properties with potenciometric titrations in water media. Chemical modification of nanotube surface were monitored with FTIR spectroscopy. Efficacy of covalent immobilization of enzymes was examined by comparing catalitical activity of immobilizied with catalitical activity of free catalase in solution. Obtained results shows that catalase can be succesfully immobilizied on titanate nanotubes, and that immobilizied catalase shows high stability and possibility of multiple usage of enzyme, with minimum lose of catalitical activity.

• Keywords: titanatne nanocjevčice; katalaza; kovalentna imobilizacija
• Keywords (english): titanate nanotubes; catalase; covalent immobilization
• Language: croatian
• URN:NBN: urn:nbn:hr:159:472614
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-30 08:55:09