Simulacija humanog gastrointestinalnog trakta pomoću "in vitro" kemostatskih i "in vitro" kontinuiranih modela

undergraduate thesis

• Title (english): Simulation of human gastrointestinal tract by "in vitro" chemostatic and "in vitro" continuous models
• Author: Stjepan Jukić
• Mentor: Jagoda Šušković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2016-06-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Razvoj mikrobiologije, biokemije, biotehnologije te molekularnih i genetičkih metoda, doveo je do povećanog istraživanja utjecaja mikroorganizama na razne procese. Od proizvodnje hrane i pića, proizvodnje bioloških goriva, razgradnje polimera, pa sve do proizvodnje antibiotika. Jedno od interesnih područja je postalo i ljudsko tijelo, odnosno kako mikroorganizmi koji se nalaze u ljudskom probavnom sustavu, utječu na procese koji se tamo odvijaju, te kakvu interakciju imaju sa domaćinom. Budući da nije etički prihvatljivo provoditi takva istraživanja na ljudima, i da je taj proces tehnički zahtjevan, razvijeni su „in vitro“ modeli probavnog sustava. Različiti modeli imaju različite funkcije samim time su pogodniji za istraživanje procesa, u različitim regijama gastrointestinalnog trakta. Cilj ovog rada je predstaviti te pet modela od kojih su tri kemostatskog tipa (EnteroMix® , Reading i SHIME), dok su tri nekemostatski kontinuirani modelo (TIM 1 i TIM 2). Objasniti ćemo na koji način pojedini model funkcionira, te ih na kraju usporediti.

Abstract (english)

The development of microbiology, biochemistry, biotechnology and molecular and genetic methods, has led to increased research of the influence of microorganisms on various processes, from food and beveragess production, production of bio-fuels, polymer degradation, to the production of antibiotics. One of the areas of interest is also the human body. Particularly how microorganisms in the human digestive system affect the processes that take place there, and how they interact with the host. Since it is not ethically acceptable to conduct such studies in humans, and because the process is technically demanding, "in vitro" models of the digestive system have been developed. Different models have specific functions and thus are more suitable for the study of processes, in sertain part of gastrointestinal tract. The aim of this paper is to present five models of which three are chemostat type (EnteroMix®, Reading and SHIME), whilethe other two have non-chenostat continuous model (TIM 1 and TIM 2). We will explain how a particular model works, and then compare them.

• Keywords: Reading; SHIME; EnteroMix; Kemostat
• Keywords (english): Reading; SHIME; EnteroMix; Chemostat
• Language: croatian
• URN:NBN: urn:nbn:hr:159:508497
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 13:47:23