Dokaz slabo patogenog soja influence ptica H5N2 lančanom reakcijom polimerazom u stvarnom vremenu (Real Time PCR)

master's thesis

• Title (english): Detection of low pathogenic avian influenza virus H5N2 using Real Time PCR
• Author: Kristina Presečki
• Mentor: Helga Medić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Meat and Fish Technology); Zagreb
• Defense date and country: 2008-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Influenca ptica danas predstavlja jednu od velikih prijetnji zdravlju čovjeka i životinja. Izvor ove bolesti primarno su ptice koje virus mogu prenjeti na velike udaljenosti što dokazuje i pojava ove bolesti diljem svijeta zadnjih godina. Važnu ulogu u prijenosu bolesti na čovjeka ima perad, naročito u zemljama Dalekog Istoka, gdje dolazi do bliskog kontakta peradi kako s ljudima tako i s divljim pticama. Poseban problem predstavlja mogućnost mutacije virusa od slabo patogenih sojeva prema visoko patogenim upravo u organizmu peradi. Stoga je vrlo važno na vrijeme dijagnosticirati bolest na nekom području, posebice u divljih ptica u neposrednoj blizini peradi kontinuiranim monitoringom, naročito u vrijeme seoba. Koristeći molekularne metode dijagnostike poput lančane reakcije polimerazom u stvarnom vremenu, praćene su smjernice WHO i Europske komisije za monitoring i dijagnostiku influence ptica. Rezultati pokazuju da su korištene metode metode izbora bilo samostalno ili u kombinaciji s klasičnim metodama izolacije na kokošjim zamecima, pri čemu je kao model korišten slabo patogeni podtip H5N2.

Abstract (english)

Avian influenza is one of the major threats for the human and animal health in the world today. Source of the virus are mainly migratory birds that can carry virus on far distances. Close contact of poultry with humans and wild birds, especially in the Far East, causes disease transmission to humans. The possibility of the virus to mutate from low to highly pathogenic strains during poultry infection makes a great difficulty and it is very important to detect this virus on time. That is done in waterfowl that is in close distance to poultry using continuous monitoring, especially during migrations. Using molecular methods of diagnostics, like Real Time PCR, guidelines of WHO and European Commission for monitoring and diagnostics are followed. The results show that chosen methods are methods of choice used alone as well as in combination with classical methods of isolation on chicken embryos, where low pathogenic avian influenza virus strain H5N2 was used as model.

• Keywords: H5N2; influenca ptica; kokošji zameci; lančana reakcija polimerazom u stvarnom vremenu; perad
• Keywords (english): avian influenza; chicken embryos; H5N2; poultry; Real Time PCR
• Language: croatian
• URN:NBN: urn:nbn:hr:159:786650
• Study programme: Title: Food Technology; specializations in: Food Engineering; Course: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer prehrambene tehnologije; smjer prehrambeno inženjerstvo (diplomirani inženjer prehrambene tehnologije; smjer prehrambeno inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-04 07:49:14