Proizvodnja mliječne kiseline s pomoću bakterije Lactobacillus amylovorus DSM 20531T šaržnim postupkom

master's thesis

• Title (english): Lactic acid production by bacterium Lactobacillus amylovorus DSM 20531T during batch cultivation
• Author: Ivana Gusić
• Mentor: Srđan Novak (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2008-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom istraživanju proveden je uzgoj bakterije Lactobacillus amylovorus DSM 20531T u kemijski definiranim podlogama s tri različita izvora ugljika (glukoza, saharoza i škrob) u laboratorijskom bioreaktoru s miješalom šaržnim postupkom. Istraživanja su provedena kako bi se utvrdila učinkovitost homolaktične fermentacije triju supstrata. Učinkovitost ovog bioprocesa procijenjena je na osnovi analize tekućinskom kromatografijom visoke djelotvornosti (HPLC). L. amylovorus DSM 20531T koristi glukozu, saharozu i škrob kao izvore ugljika. Ova bakterija u podlozi s škrobom ima kratku fazu prilagodbe. L. amylovorus DSM 20531T simultano razgrađuje škrob i proizvodi mliječnu kiselinu uz produktivnost bioprocesa od 1.1 g L-1 h-1 i specifičnu brzinu rasta od 0.4 h-1. Usporedba biokinetičkih parametara šaržnog uzgoja L. amylovorus DSM 20531T pokazuje da bi se uz primjenu ove bakterije bioproces proizvodnje mliječne kiseline iz škroba i škrobnih supstrata mogao učinkovito i ekonomski povoljno primjeniti u industrijskom mjerilu.

Abstract (english)

In this investigation a batch cultivation of bacterium Lactobacillus amylovorus DSM 20531T was conducted in chemicaly defined media with three different carbon sources (glucose, sucrose and starch) in a lab-scale stirred tank bioreactor. The investigation was performed in order to define efficiency of homolactic fermentation of the three substrates. The estimation of the bioprocess efficiency was based on high-pressure liquid chromatography (HPLC) analysis. L. amylovorus DSM 20531T grew well on glucose, sucrose and starch. In the starch-containing medium lag phase was short. L. amylovorus DSM 20531T simultaneously degrades starch and produces lactic acid with productivity of 1.1 g L-1 h-1 and specific growth rate of 0.4 h-1. A comparison of the biokinetic parameters of batch cultivation of L. amylovorus DSM 20531T indicates that an efficient and cost-effective production of lactic acid on a industrial scale could be implemented.

• Keywords: mliječna kiselina; šaržni uzgoj; Lactobacillus amylovorus; različiti supstrati; HPLC
• Keywords (english): lactic acid; batch cultivation; Lactobacillus amylovorus; different substrates; HPLC
• Language: croatian
• URN:NBN: urn:nbn:hr:159:812614
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-04 11:51:07