Utjecaj različitih vrsta sirovina i količina hranjivih soli na umnožavanje kvasca Saccharomyces cerevisiae kod proizvodnje rafiniranog etanola

master's thesis

• Title (english): Effect of different types of raw material and amount of nutrients salts on multiplication of yeast Saccharomyces cerevisiae in the production of rafined ethanol
• Author: Dragana Nišević
• Mentor: Vesna Stehlik-Tomas (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2008-03-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu je ispitivan utjecaj različitih sirovina i koncentracija hranjivih soli u podlozi na rast kvaščeve biomase i prinos etanola s kvascem Saccharomyces cerevisiae. Korištene su sirovine: melasa, glukozni sirup, otopina šećera i ošećerena kukuruzna komina. Fermentacije su provedene šaržno u semiaerobnim uvjetima pri 30˚C. Najveći prirast kvaščeve biomase (41,6 g/L) i prinos etanola (6,21% vol/vol) dobiven je kultivacijom na ošećerenoj kukuruznoj komini sa hranjivim solima (NH4)2HPO4 i (NH4)2SO4. Najmanji prirast kvaščeve biomase (6,49 g/L) i prinos etanola (2,34% vol/vol) je dobiven uzgojem na glukoznom sirupu sa hranjivim solima (NH4)2HPO4 i (NH4)2SO4 bez održavanja pH.

Abstract (english)

Present work is based on the observation of the effect of different raw matirial and concentration of nutrient salts in the cultivation medium, on the fermentation in the production of yeast biomasses and ethanol by Saccharomyces cerevisiae. The raw matirial that have been used are: molasses, glucose syrup, solution of sugar and corn starch hydrolysate. Fermentations were conducted in semiaerobic batch cultures at 30˚C .The best biomass yield (41,6 g/L) and alcohol yield (6,21% vol/vol) was achieved by cultivation on corn starch hydrolysate with nutrient salts (NH4)2HPO4 i (NH4)2SO4. The smolest biomass yield (6,49 g/L) and alcohol yield (2,34% vol/vol) was achieved by cultivation on glucose syrup with nutrient salts (NH4)2HPO4 i (NH4)2SO4 without maintaining pH-value.

• Keywords: S. cerevisiae; melasa; glukozni sirup; otopina šećera; škrob
• Keywords (english): S. cerevisiae; molasses; glucose syrup; solution of sugar; corn
• Language: croatian
• URN:NBN: urn:nbn:hr:159:903273
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-05 08:00:43