| Abstract | Citotoksički učinak herbicida atrazina i protektivan učinak vitamina E praćen je na staničnoj liniji bubrega 1 dan starih hrčaka (BHK 21 C13). BHK 21 C13 stanice rasle su u suspenziji na 37˚C u atmosferi 95% zraka i 5% CO2, u mediju za uzgoj Dulbecco's MEM uz dodatak 5% NCS-a (Newborn Calf Serum). Početna koncentracija stanica iznosila je 1,77x105 stanica/mL medija za uzgoj. Tijekom 72 sata metodom Trypan Blue praćen je citotoksički učinak atrazina u koncentraciji 20, 40, 60, 80, 120 i 160 μg/mL medija za uzgoj i učinak vitamina E u koncentraciji 25 i 50 μg/mL medija za uzgoj. Proliferacija BHK 21 C13 stanica uz dodatak vitamina E u koncentraciji 25 i 50 μg/mL medija za uzgoj nakon 72 sata poboljšana je za 5,71%, odnosno 10,41% u odnosu na kontrolnu vrijednost. Pri koncentraciji atrazina od 20, 40, 60, 80, 120 i 160 μg/mL medija za uzgoj inhibicija rasta iznosi 27,99%, 37,88%, 45,22%, 62,29%, 68,43% i 76,61%. Pri istim koncentracijama atrazina uz dodatak vitamina E u koncentraciji 25 μg/mL medija za uzgoj inhibicija rasta iznosi 27,62%, 39,36%, 49,03%, 60,36%, 61,05%, 76,66%. Uz dodatak vitamina E u koncentraciji 50 μg/mL medija za uzgoj pri koncentraciji atrazina od 20, 40, 60, 80, 120 i 160 μg/mL medija za uzgoj inhibicija rasta iznosi 17%, 26,88%, 36,96%, 51,98%, 62,06% i 70,75%. Vitamin E u koncentracijama 25 i 50 μg/mL medija za uzgoj utječe na dinamiku i proliferaciju BHK 21 C13 stanica. Uz prisutnost vitamina E proliferacija BHK 21 C13 stanica je bila bolja i dokazan je protektivni učinak na stanicama tretiranim atrazinom. |
| Abstract (english) | Cytotoxic effect of herbicide atrazine and protective influence of vitamin E were followed on cell line from one day old hamster kidney (BHK 21 C13 cell line). BHK 21 C13 cells were cultivated at 37˚C in the atmosphere of 95% air and 5% CO2, in the medium Dulbecoo´s MEM with 5% NCS (Newborn Calf Serum). The initial concentration of cells was 1,77 x 105 cells/mL in cultivating medium. The cytotoxic effect of atrazine in concentration of 20, 40, 60, 80, 120 and 160 μg/mL in cultivating medium, as well as the effect of vitamin E in concentration of 25 and 50 μg/mL of cultivating medium was followed by Trypan Blue exclusion method during 72 hours. Proliferation of BHK 21 C13 cells with the addition of vitamin E at concentration of 25 and 50 μg/mL of cultivating medium, after 72 hours was improved for 5.71%, that is 10.41% compared to control value. At atrazine concentration of 20, 40, 60, 80, 120 and 160 μg/mL of cultivating medium growth inhibition was 27.99%, 37.88%, 45.22%, 62.29%, 68.43% and 76.61%. At the same atrazine concentrations, with the addition of vitamin E at concentration of 25 μg/mL of cultivating medium, growth inhibition was 27.62%, 39.36%, 49.03%, 60.36%, 61.05% and 76.66%. With the addition of vitamin E at concentration of 50 μg/mL of cultivating medium at atrazine concentration of 20, 40, 60, 80, 120 and 160 μg/mL of cultivating medium growth inhibition was 17%, 26.88%, 36.96%, 51.98%, 62.06% and 70.75% respectively. Vitamin E in concentrations of 25 and 50 μg/mL of cultivating medium has an effect on dynamic and better proliferation of BHK 21 C13 cells. During the presence of vitamin E proliferation of BHK 21 C13 cells was proven as well as the protective effect during the cells treatment with atrazine. |
