Karakterizacija beta-1,3-glukana izoliranog različitim metodama iz kvasca Saccharomyces cerevisiae

master's thesis

• Title (english): Characterization of beta-1,3-glucan isolated by diferent methods from yeast Saccharomyces cerevisiae
• Author: Petra Galović
• Mentor: Vesna Zechner-Krpan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2007-04-04, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

β-glukan, homopolimer glukoze, ima sposobnost regulacije imunološkog sustava. Stanična stijenka pivskog kvasca Saccharomyces cerevisiae dostupan je izvor β-1,3-glukana. Glukan iz kvasca zaostalog nakon proizvodnje piva je izoliran trima različitim metodama, te osušen: na zraku, liofilizacijom i raspršivanjem. U tako dobivenim preparatima β-glukana određivana je veličina čestica, kapacitet vezanja vode i ulja, sposobnost bubrenja, biološka aktivnost, elektronskom mikroskopijom utvrđena im je mikrostruktura, te je napravljena FT-IR spektroskopija za karakterizaciju glukana.

Abstract (english)

β-glucan, a homopolymer of glucose, is well-known regulator of immune system. The cell-wall of yeast Saccharomyces cerevisiae is an available source of β-1,3-glucan. Glucan was isolated from brewer΄s yeast with three different methods and dried on air, in a lyophilisator and in spray-drying chamber. In such obtained β-glucan preparations dimension of particles, water and oil apsorption capacities, swelling capabilities and biological activity were measured. Microstructure of glucan was analysed by electronic microscopy and characterized by FT-IR spectroscopy.

• Keywords: β-glukan; pivski kvasac; Saccharomyces cerevisiae
• Keywords (english): β-glucan; brewer's yeast; Saccharomyces cerevisiae
• Language: croatian
• URN:NBN: urn:nbn:hr:159:614117
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-06 09:43:59