Citotoksički učinak lindana na dinamiku rasta CHO-K1 (Chinese Hamster Ovary) stanica primjenom metoda Trypan Blue i Kenacid Blue

master's thesis

• Title (english): Cytotoxic effects of lindane on CHO-K1 (Chinese Hamster Ovary) cell growth followed by Trypan Blue and Kenacid Blue methods
• Author: Teuta Murati
• Mentor: Jasna Kniewald (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2006-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Prisutnost ostataka pesticida u hrani, vodi i tlu predstavlja opasnost za cjelokupnu floru i faunu, a time i potencijalnu opasnost na zdravlje čovjeka. Toksični učinci pesticida utvrđuju se provođenjem klasičnih (in vivo) i alternativnih (in vitro) testova toksičnosti. U ovom radu kao test-sustav za praćenje citotoksičkog učinka lindana primjenjena je stanična linija ovarija kineskog hrčka (CHO-K1) u monosloju, uzgojena u Dulbecco MEM mediju uz dodatak 5% NCS-a (Newborn Calf Serum), u atmosferi 95% zraka i 5% CO2 na temperaturi 37°C. Citotoksički učinak lindana u CHO-K1 staničnoj liniji početne koncentracije 2 x 104 stanica/mL medija za uzgoj ispitan je u rasponu koncentracija od 20-100 µg lindana/mL medija za uzgoj primjenom metoda Trypan Blue i Kenacid Blue. IC50 vrijednosti izračunate su iz grafičkog prikaza ovisnosti % inhibicije o logaritmu upotrebljene koncentracije lindana. IC50 vrijednost određena Trypan Blue metodom iznosi 35,34 µg lindana/mL medija za uzgoj, a Kenacid Blue metodom 74,08 µg lindana/mL medija za uzgoj. Primjenjene metode su brze, jednostavne za izvođenje i primjenjive u ispitivanju citotoksičnosti ksenobiotika u CHO-K1 staničnoj liniji.

Abstract (english)

The presence of pesticide residues in food, water and soil represents a risk for the entire flora and fauna and a potentional risk for human health. Determinations of pesticide toxic effects are carried out by classical (in vivo) and alternative (in vitro) toxicity tests. In this work as a test-system for the determination of cytotoxic effect of lindane on chinese hamster ovary cell line (CHO-K1) in monolayer was used, cultivated in Dulbecco's MEM with addition of 5% NCS (Newborn Calf Serum), in atmosphere of 95% air and 5% CO2 at 37°C. Cytotoxic effects of lindane in concentration range of 20-100 µg lindane/mL of cultivating medium was examined in CHO-K1 cell line with initial concentration of 2 x 104 cells/mL of cultivating medium by Trypan Blue and Kenacid Blue methods. IC50 was determined from the slope of % inhibition vs. log dose values. IC50 value determined by Trypan Blue method is 35,34 µg lindane/mL of cultivating medium, and determined by Kenacid Blue method is 74,08 µg lindane/mL of cultivating medium. Applied methods are fast, easy to realize and applicable for the performing of the testing of xenobiotics cytotoxicity in CHO-K1 cell line.

• Keywords: CHO-K1 stanična linija; citotoksičnost; lindan; Kenacid Blue; Trypan Blue
• Keywords (english): CHO-K1 cell line; cytotoxicity; lindane; Kenacid Blue; Trypan Blue
• Language: croatian
• URN:NBN: urn:nbn:hr:159:488261
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-07 11:29:41