Utjecaj karvona i tanina na biološku razgradnju polikloriranih bifenila

master's thesis

• Title (english): Effect of carvone and tanin on biological degradation of polychlorinated biphenyls
• Author: Željka Vujaković
• Mentor: Margareta Glancer-Šoljan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for the Biological Waste Water Treatment); Zagreb
• Defense date and country: 2006-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U istraživanju su primijenjene dvije mješovite kulture (TSZ7 i JEL), porijeklom iz tla zagađenog PCB-ima. Obje mješovite bakterijske kulture uspješno su rasle u mineralnoj podlozi s bifenilom kao jedinim izvorom ugljika i energije. Istraživanja biološke razgradnje PCB-a provedena su tijekom submerznog uzgoja mješovitih bakterijskih kultura u mineralnoj podlozi uz dodatak komercijalne smjese Aroclor 1248 i odabranih inducera (bifenil, karvon, tanin). Biološka transformacija komercijalne smjese PCB-a, Aroclor 1248 uspješno je provedena s obje kulture te sa sva tri inducera. Rezultati su pokazali da osim bifenila, koji se potvrdio kao najučinkovitiji inducer, karvon i tanin također induciraju enzimski sustav za razgradnju PCB-a. Bez obzira na učinkovitost bifenila, zbog njegovih štetnih učinaka na okoliš i zdravlje čovjeka, biološke transformacije PCB-a inducirane biljnim metabolitima karvonom i taninom, imat će prednost u primjeni za ubrzanje biotransformacijskih procesa u tlu zagađenom PCB-ima.

Abstract (english)

Two bacterial mixed cultures (TSZ7 and JEL), isolated from long time PCB-contaminated soil were enriched by growing on biphenyl as the sole carbon and energy source and as inducer of PCB-degradation pathway. During the microcosm enrichment both cultures grew readily on biphenyl. Furthermore, PCB biodegradation experiments were performed in the presence of biphenyl as well known inducer and in the presence of two plant-derived compounds, carvone and tanin, as potential inducers of PCB-degradation pathway. Results obtained after three-week biodegradation experiment with the commertial mixture Aroclor 1248 in the presence of all tested inducers suggested substantial PCB-degrading activity of both cultures. As expected the best effectiveness was observed for both cultures in the presence of biphenyl, however carvone and tanin as potential inducers also exerted an enhancing effect on PCB degradation. Therefore those two plant compounds were designated as promising candidates for use in bioremediation of PCB contaminated soil.

• Keywords: aerobna razgradnja PCB-a; bifenil; karvon; PCB; tanin
• Keywords (english): aerobic transformation of PCB; biphenyl; carvone; PCB; tanin
• Language: croatian
• URN:NBN: urn:nbn:hr:159:021978
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-07 11:45:35