Analiza sadržaja aktivne komponente u ceturoksim-natrij-injekcijama HPLC i HPCE metodom – usporedba metoda

master's thesis

• Title (english): Analysis of main component content in Cefuroxim-sodium injections using HPLC and HPCE- method comparison
• Author: Ivana Kurteš
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); Zagreb
• Defense date and country: 2005-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kapilarnom elektroforezom (CE) i tekućinskom kromatografijom visoke djelotvornosti (HPLC) analizirane su Cefuroksim-natrij injekcije i na temelju rezultata uspoređene su te dvije separacijske metode. Određeni su parametri preciznosti, točnosti, osjetljivosti, linearnosti, robustnosti te drugih fizikalnih parametara koji utječu na efikasnost separacije. Rezultati su pokazali veću predanost izvođenja analize HPLC metodom. Analiziranjem uzoraka u višim koncentracijama i uporaba odgovarajućih internih standarda može poboljšati validacijske aspekte kapilarne elektorforeze.

Abstract (english)

The main component content in Cefuroxim-sodium injections was analyzed by using Capillary Electrophoresis (CE) and High Performance Liquid Chromatography (HPLC) and the methods were compared on the basis of the results obtained. Parameters such as precision, accuracy, sensitivity, linearity, robustness and other physical characteristics that affect separation efficiency were determined. The results have shown the advantage of HPLC method upon CE method. The analysis of samples in high concentrations and the use of an appropriate internal standard can improve validation aspects in CE method.

• Keywords: cefuroksim-natrij; kapilarna elektroforeza; tekućinska kromatografija visoke djelotvornosti; separacija
• Keywords (english): Capillary Electrophoresis; High Performance Liquid Chromatography; Cefuroxim-sodium; separation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:465067
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-11 13:04:20