Učinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida

undergraduate thesis

• Title (english): The effect of 5-hydroxymethyl furfuryl and furfuryl on growth of fungus Mortierella isabellina and lipid synthesis
• Author: Manda Madaras
• Mentor: Mirela Ivančić - Šantek (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2017-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Oleaginozni mikroorganizmi proizvode lipide koji se mogu koristiti kao zamjenska sirovina za proizvodnju biodizela umjesto biljnih ulja. Oleaginozna plijesan Mortierella isabellina nakuplja triacilglicerole kao stanične rezervne lipide čiji udjel u suhoj tvari biomase iznosi i do 80 %. Lignocelulozna biomasa izvor je jeftinih ugljikohidrata koji se mogu koristiti kao izvor ugljika za rast mikroorganizma i sintezu proizvoda (npr. bioetanola). Procesom predobrade poboljšava se enzimska razgradnja lignocelulozne sirovine s celulazama te se povećava prinos reducirajućih šećera nastalih hidrolizom. Međutim tijekom ovog procesa nastaje niz nusproizvoda iz lignocelulozne sirovine koji inhibiraju radni mikroorganizam i celulolitičke enzime. U ovom radu istražen je učinak furfurala i 5-hidroksimetil furfurala (HMF) na rast i proizvodnju lipida s plijesni M. isabellina. Rezultati pokazuju da furfural jače inhibira rast i sintezu lipida od HMF-a. Rast plijesni i sinteza lipida je potpuno inhibirana pri koncentraciji furfurala većoj od 0,6 g/L i HMF-a većoj od 2 g/L.

Abstract (english)

Lipids produced by oleaginous microorganisms can be used as alternative feedstock for production of biodiesel instead of plant oils. Oleaginous fungus Mortierella isabellina has the ability to accumulate triacylglycerols, as cellular storage lipids, up to 80% of the biomass. Lignocellulosic biomass is source of cheap carbohydrates that can be used as carbon source for the microbial growth and product synthesis (e.g. bioethanol production). The pretreatment process improves enzymatic digestibility of lignocellulosic biomass by cellulases and enhanced the yield of reducing sugars by hydrolysis. However, during this process many lignocellulose-derived by-products are formed that inhibit microorganism and cellulolic enzymes. The influence of furfural and 5-hydroxymethyl furfural (HMF) on the growth and lipid production by fungus M. isabellina was investigated. The results indicate that furfural inhibits the growth and lipid synthesis markedly more than HMF. The growth and lipid accumulation of fungus was completely inhibited at concentration of furfural and HMF higher than 0,6 g/L and 2 g/L, respectively.

• Keywords: Mortierella isabellina; lipidi; biodizel; lignoceluloza; inhibitor
• Keywords (english): Mortierella isabellina; lipids; biodiesel; lignocellulose; inhibitor
• Language: croatian
• URN:NBN: urn:nbn:hr:159:040504
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-18 08:18:18