Utjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata

master's thesis

• Title (english): The effect of deep eutectic solvents on lipase catalyzed hydrolysis of (R,S)-1-phenylethyl-acetate
• Author: Martina Vukšić
• Mentor: Marina Cvjetko Bubalo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2017-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U svrhu uspostavljanja visokoučinkovitih procesa proizvodnje industrijski važnih kemikalija, u posljednjih nekoliko godina eutektička otapala intenzivno se proučavaju kao zelena zamjena za škodljiva otapala u procesima organske sinteze i biokatalize. U ovom radu ispitana je mogućnost primjene lipaze B kao biokatalizatora u hidrolizi (R,S)-1-feniletil-acetata u kolinijevim eutektičkim otapalima. Uspoređujući sve ispitane parametre (η, VP, ee i VE), zaključuje se da su eutektička otapala superiornija za lipazom kataliziranu hidrolizu od konvencionalnih organskih otapala, pri čemu se otapalo ChGlc s 50 % vode pokazalo kao najbolje. U istoimenom otapalu kao i u puferu, ispitana je termička stabilnost lipaze kroz vremenski period od 72h u temperaturnom rasponu od 20-80 °C. Pri tome je ustanovljeno da u tercijarnim mješavinama DES-a ChGlc nakon 72 h inkubacije, najveći postotak inicijalne aktivnosti lipaza zadržava pri temperaturi od 40 °C. Osim toga, ustanovljeno je da lipaza pokazuje veću termičku stabilnost u eutektičkim otapalima nego u puferu.

Abstract (english)

For the purpose of establishing new sustainable processes for the production of industrially important compounds, in recent years deep eutectic solvents have been intensively studied as a possible replacement for organic solvents for biocatalytic and organic reactions. Within this work, the hydrolysis of (R,S)-1-phenylethyl acetate within cholinium-based deep eutectic solvents was studied. Comparing all the tested parameters (η, VP, ee and VE), it is clear that deep eutectic solvents are superior to conventional organic solvents for lipase catalyzed hydrolysis, where solvent ChGlc with 50 % of water was found to be the best between all used. Thermal stability of lipase at different temperatures through a certain period of time, was also examined. It was established that after 72 hours of incubation in tertiary DES mixtures, lipase retains its higest activity at 40 °C. Thermal stability of lipase was proven to be higher in deep eutectic solvents than in buffer.

• Keywords: biokataliza; lipaza; eutektička otapala; (R; S)-1-feniletil-acetat; termička stabilnost
• Keywords (english): biocatalysis; deep eutectic solvents; (R; S)-1-phenylethyl-acetate; thermal stability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:609397
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-08 10:08:56