Izolacija bioaktivnih spojeva iz lanene pogače pomoću novih tehnologija i eutektičkih otapala

master's thesis

• Title (english): Isolation of bioactive compounds from flaxseed cake with novel method and eutectic solvents
• Author: Bruna Rakuljić
• Mentor: Dubravka Škevin (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Oil and Fat Technology); Zagreb
• Defense date and country: 2017-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Visoka koncentracija nutritivno vrijednih polifenolnih spojeva čine pogaču lana jako dobrom sirovinom za njihovu ekstrakciju. Cilj ovog rada bilo je izolirati i odrediti bioaktivne spojeve iz pogače lana upotrebom eutektičkih otapala i novih metoda ekstrakcije, kao bržih i ekološki prihvatljivijih postupaka. Kao najoptimalnije eutektičko otapalo pokazao se kolin klorid:urea:glicerol, a najveći udio fenolnih spojeva je ekstrahiran kombinacijom mikrovalova i ultrazvuka. Mikrovalnom ekstrakcijom detektirani su: sekoizolarikirezinol-diglukozid (SDG) (2.35 mg g-1), glukozid p-kumarinske kiseline (GPK) (4.13 mg g-1) te glukozid ferulinske kiseline (GFK) (1.94 mg g-1). Uz mikrovalno-ultrazvučno potpomognutu ekstrakciju, maksimalne izolirane koncentracije za SDG bile su 3.25 mg g-1, za GPK 4.34 mg g-1 i GFK 2.35 mg g-1, odnosno uspješno je ekstrahirano 15 % SDG-a, 42 % GPK-a te 45 % GFK-a u odnosno na koncentracije dobivene referentnom metodom, ali uz značajno kraće vrijeme trajanja procesa ekstrakcije. Potrebna su daljnja istraživanja u svrhu odabira najpogodnijeg eutektičkog otapala i optimiranja procesa ekstrakcije.

Abstract (english)

High concentrations of bioactive polyphenolic compounds make flaxseed cake very good raw material for their extraction. The aim of this paper was to isolate and determine bioactive compounds from flaxseed cake using eutectic solvents and new extraction methods as faster and more environmentally friendly methods. Choline chloride: urea: glycerol was found as the most optimal eutectic solvent, and the higher yield of the phenolic compounds were extracted by a combination of microwaves and ultrasound. Using only microwave extraction the highest obtained results were: secoizolariciresinol-diglucoside (SDG) (2.35 mg g-1), p-coumaric acid glucoside (GPK) (4.13 mg g-1) and ferulic acid glucoside (GFK) (1.94 mg g-1). With ultrasonic microwave-assisted extraction, the maximum isolated concentration of SDG was 3.25 mg g-1, GPK 4.34 mg g-1 and GFK 2.35 mg g-1.Compared with reference method, 15% SDG was successfully extracted, 42% of GPK and 46% of GFK, but with significantly shorter processing time. Further research is required to select the most suitable eutectic solvent and optimize the extraction process.

• Keywords: lanena pogača; sekoizolarikirezinol-diglukozid; fenolne kiseline; eutektička otapala; Mikrovalno-ultrazvučna ekstrakcija
• Keywords (english): flaxseed cake; secoizolaricirezinol diglucoside; phenolic acid; deep eutectic solvents; ultrasonic-microwave assisted extraction
• Language: croatian
• URN:NBN: urn:nbn:hr:159:452684
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-09 09:55:53