Optimizacija postupka pripreme uzorka ribe za detekciju histamina SERS-om

master's thesis

• Title (english): Optimization of fish processing process preparation for histamine detection with SERS method
• Author: Daniela Lončar
• Mentor: Sanja Vidaček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Meat and Fish Technology); Zagreb
• Defense date and country: 2017-07-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Histamin je najvažniji biogeni amin koji pri povišenoj koncentraciji u hrani morskog podrijetla predstavlja toksikološki rizik. Ramanova spektroskopija se pokazala brzom i invazivnom metodom za detekciju histamina, dok su tradicionalne metode skuplje i složenije te teže primjenjive u industrijskim uvjetima. Cilj ovog istraživanja bio je pročistiti ekstrakte različitim otapalima kako bi se histamin izdvojio iz vodenog sloja i bolje detektirao SERS (površinski pojačano Ramanovo raspršenje) metodom, te time izbjeći dugi postupak uparavanja i skratiti navedenu metodu. Koeficijent koleracije (R2= 0,9614) i intenzitet vrpce histamina na 1570 cm-1 pokazali su se najboljima kod ekstrakta B1 (ekstrakcija HCl-om), dok je kod ekstrakta B2 (ekstrakcija s izooktanom i vodom) najveća točnost metode (95,45%) kod vrpce histamina na 1264 cm-1. Zbog otežanih uvjeta u radu s ekstraktima B i C (ekstrakcija 0.1 i 0.5M NaOH), te tijekom dodavanja i miješanja isptivanih otapala (HCl, izooktan, cikloheksan), može se zaključiti da te postupke nije preporučljivo primjenjivati.

Abstract (english)

Histamine is the most important biogenic amine which represents a toxicological risk at elevated concentrations in sea food. Raman spectroscopy proved to be a fast and invasive histamine detection method, while traditional methods are more expensive, complex and difficult to apply in industrial conditions. The aim of this study was to purify the extracts with different solvents in order to separate histamine from the aqueous layer and enable better detection by the SERS (Surface Enhanced Raman Scattering) method. Thus the relatively long evaporation process was to be avoided and the method would shorten. The correlation coefficient c (R2= 0.9614) and the histamine band intensity at 1570 cm--1 were the best for analysing extract B1 (extraction with HCl). The highest method accuracy (95.45%) in extract B2 (extraction with isooctane and water) was achieved by the histamine band at 1264 cm-1. Due to difficulties in analysing extracts B and C (extraction with 0.1 and 0.5 M NaOH), and during the addition and mixing with investigated solvents (HCl, isooctane and cyclohexane) such procedures are not recommended for use.

• Keywords: riba; histamin; Ramanova spektroskopija; površinski pojačano Ramanovo raspršenje (SERS); koeficijent koleracije
• Keywords (english): fish; histamine; Raman spectroscopy; Surface Enhanced Raman Scattering (SERS); correlation coefficient
• Language: croatian
• URN:NBN: urn:nbn:hr:159:664826
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-09 10:36:08