Proizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima

master's thesis

• Title (english): Bioethanol production on the mild acid hydrolysates of wheat straw obtained by phosphoric acid at various scales
• Author: Maja Galić
• Mentor: Božidar Šantek (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2017-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bioetanol je važan obnovljiv izvor energije koji ima veliki potencijal za zamjenu fosilnih transportnih goriva. Velike količine jeftinih industrijskih i poljoprivrednih ostataka idealne su sirovine za proizvodnju bietanola pri čemu se njihovim zbrinjavanjem sprječava zagađenje okoliša. Pšenična slama je dobar primjer takve sirovine koji se može primjeniti u procesu proizvodnje bioetanola. Međutim, pšenična slama mora proći proces predobrade da bi se mogla primjeniti u procesu proizvodnje bioetanola. U ovom istraživanju proučavan je proces blago kisele hidrolize pomoću fosforne kiseline u visokotlačnom reaktoru pri različitim kombinacijama parametara procesa predobrade. Proučavane su različite kombinacije svih parametara procesa predobrade: temperatura (160 – 200 °C), vrijeme zadržavanja (1 - 10 minuta) i koncentracije fosforne kiseline (0,5 – 2,0 %). Najveća koncentracija fermentabilnih šećera zabilježena je u tekućem dijelu hidrolizata pšenične slame kod predobrade na 180 °C, vremenu zadržavanja od 10 minuta i koncentraciji H3PO4 od 2,0 %. U čvrstom dijelu hidrolizata pšenične slame kod prethodno navedenih uvjeta detektirani su ovi sastojci: glukan (42,43 %), ksilan (19,64 %), arabinan (9,01 %). Dobiveni hidrolizati pšenične slame korišteni su za anaerobni uzgoj divljeg i genetički modificiranog soja (Y9) kvasca S. cerevisiae u Erlenmeyer tikvicama i horizontalno rotirajućem cijevnom bioreaktoru. Najuspješniji anaerobni uzgoj genetički modificiranog soja kvasca S. cerevisiae Y9 proveden je na hidrolizatu pšenične slame dobivenom pri temperaturi od 180 °C, vremenu zadržavanja od 10 minuta i koncentraciji fosforne kiseline od 2,0 % u HRCB-u pri čemu su dobiveni ovi pokazatelji uspješnosti bioprocesa: Pr: 0,0057 gL-1h-1, YP/S: 0,058 gg-1, E: 11,34 %.

Abstract (english)

Bioethanol is an important renewable energy source that has great potential to replace fossil transportation fuels. Large quantities of cheap industrial and agricultural residues are the ideal raw materials for the production of bioethanol, whereby their disposal prevents environmental pollution. Wheat straw is a good example of such raw materials that can be applied in the production process of bioethanol. However, wheat straw has to go through the process of pretreatment that could be applied in the production process of bioethanol. In this study, the process of mild acid hydrolysis by phosphoric acid in a high - pressure reactor at various combinations of processing parameters was studied. Various combinations of all processing parameters: temperature (160 - 200 °C), holding time (1 - 10 minutes) and phosphoric acid concentration (0.5 - 2.0%) were studied. The highest concentration of fermentable sugar was observed in the liquid hydrolyzate of wheat straw pretreated at 180 °C, residence time of 10 minutes and a concentration of 2,0 % H3PO4. In the solid hydrolyzate of wheat straw, at previous conditions, these components are detected: glucan (42,43 %), xylan (19,64 %), arabinan (9,01 %). The obtained hydrolyzate of wheat straw was used for anaerobic cultivation of wild and genetically modified yeast S. cerevisiae, Y9 in Erlenmeyer flasks and horizontally rotating tubular bioreactor. The most successful anaerobic cultivation of genetically modified yeast S. cerevisiae, Y9 was carried out on the hydrolyzate of wheat straw obtained at 180 °C, 10 minute retention time and 2.0% phosphoric acid concentration in HRCB, where these bioprocess performance indicators were obtained: Pr: 0.0057 gL-1h-1, YP / S: 0.058 gg-1, E: 11.34%.

• Keywords: bioetanol; pšenična slama; blago kisela predobrada; visokotlačni bioreaktor; horizontalno rotirajući cijevni bioreaktor; različiti sojevi kvasca; Saccharomyces cerevisiae
• Keywords (english): bioethanol; wheat straw; mild acidic pretreatment; high - pressure bioreactor; horizontally rotating tubular bioreactor; different strains of yeast; Saccharomyces cerevisiae
• Language: croatian
• URN:NBN: urn:nbn:hr:159:641134
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-23 14:24:50