Proizvodnja bioetanola iz lignoceluloznog otpada s pomoću fungalnih ko-kultura

master's thesis

• Title (english): Bioethanol production from lignocellulose waste using fungal co-cultures
• Author: Dina Kačar
• Mentor: Anita Slavica (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2016-10-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Dva filamentozna funga, Phlebia acerina FBCC4 i Phlebia radiata FBCC43, odabrani su kao biokatalizatori za hidrolizu lignoceluloze do reducirajućih šećera i fermentaciju ovako proizvedenih reducirajućih šećera do etanola. Bioproces direktne konverzije lignoceluloze u etanol proveden je s pomoću monokultura dvaju funga kao i ko-kulture ovih dvaju funga FBCC4 i FBCC43. Eksperimenti su provedeni u modificiranoj Okamoto podlozi u kojoj je dodan reciklirani karton (50 g L-1) kao glavni izvor ugljika i energije. Bioproces je poboljšan korištenjem ko-kulture P. acerina FBCC4, P. radiata FBCC43 i odabranog kvasca Saccharomyces cerevisiae HAMBI1164 (FBCC4 + FBCC43 + HAMBI1164). Ovaj kvasac dobar je kandidat za ko-kulturu FBCC4 + FBCC43 + HAMBI1164 jer je fermentirao glukozu i vrlo učinkovito proizveo etanol. Nadalje, ovaj je kvasac dobro rastao kao član ko-kulture FBCC4 + FBCC43 + HAMBI1164 i na njegov rast skoro da nisu utjecali oksalna kiselina ili proizvodi metabolizma FBCC4. Ko-kultura FBCC4 + FBCC43 + HAMBI1164 proizvela je najveću koncentraciju etanola (redom 0,34 g L-1 i 1,89 g L-1) nakon 9 dana bioprocesa pri mikroaerofilnim i skoro anaerobnim uvjetima. Tijekom ovog perioda bioprocesa masa kartona se smanjila za 27,1 % od početne mase, dok je masa micelija porasla do 0,19 g u jednoj Erlenmeyer tikvici.

Abstract (english)

Two filamentous white-rot fungi, Phlebia acerina FBCC4 and Phlebia radiata FBCC43, were employed as biocatalysts for hydrolysis of lignocellulose to reducing sugars and fermentation of reducing sugars therefrom to ethanol. Bioprocess of direct conversion of lignocellulose to ethanol was carried out by using monocultures of the two fungi as well as two-member co-culture of FBCC4 and FBCC43. Experiments were performed in modified Okamoto medium with core board (50 g L-1) as main carbon and energy source. The bioprocess was improved by employing three-species co-culture of P. acerina FBCC4, P. radiata FBCC43 and selected yeast Saccharomyces cerevisiae HAMBI1164 (FBCC4 + FBCC43 + HAMBI1164). The yeast was a good candidate for the three-species co-culture because it was able to ferment glucose and produce ethanol very efficiently. In addition, the yeast grew well as a member of FBCC4 + FBCC43 + HAMBI1164 and its growth was almost not affected by oxalic acid or products of metabolism of FBCC4. Three-species co-culture FBCC4 + FBCC43 + HAMBI1164 produced the highest concentration of ethanol (0.34 g L-1 and 1.89 g L-1) on the 9th day of the bioprocess under microaerophilic and almost anaerobic conditions, respectively. During this period weight of core board was reduced for 27.1 % of the initial weight while weight of dry mycelial biomass increased to 0.19 g in one Erlenmeyer flask.

• Keywords: lignoceluloza; fungi; etanol; ko-kulture
• Keywords (english): lignocellulose; fungi; ethanol; co-cultures
• Language: english
• URN:NBN: urn:nbn:hr:159:605740
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-25 13:21:53