Priprava ekstrakata komine grožđa pomoću eutektičnih otapala, izolacija antocijana te njihova in vitro biološka aktivnost

master's thesis

• Title (english): Preparation of grape pomace extracts by using deep eutectic solvents, isolation of anthocyanins and their in vitro biological activity
• Author: Manuela Panić
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2016-06-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Eutektična otapala (DES), kao nova zelena otapala, posljednjih se godina istražuju kao alternativa za zelenu ekstrakciju i separaciju antocijana iz komine grožđa. U ovom radu pripremljeno je 5 različitih DES-ova s ciljem pronalaženja najboljeg otapala za ekstrakciju antocijana. Najveći učinak ekstrakcije postignut je s eutektičnim otapalom kolin klorid:prolin:jabučna kiselina. Iz pripravljenih ekstrakata antocijani su izolirani na makroporoznoj smoli Amberlite XAD-16 i eluirani 96%-tnim etanolom. Učinak etanolnih eluata ispitan je u in vitro uvjetima na tri stanične linije (HeLa, MCF-7 i HEK293T), a najizraženiji citotoksični učinak je vidljiv kod etanolnog eluata betain:jabučna kiselina. Određen je i antioksidacijski kapacitet etanolnih eluata i najvišu vrijednost ima etanolni eluat betain:jabučna kiselina. Razvijen postupak ekstrakcije i izolacije na makroporoznoj smoli pokazao se kao alternativna i zelena metoda za ekstrakciju i koncentriranje antocijana iz komine grožđa.

Abstract (english)

Deep eutectic solvents (DES), as new extraction solvents, have attracted increasing attention for the green and efficient extraction and separation of anthocyanins from grape pomace. In this study, five different DESs were prepared and observed to determine which of them possess the predominant extraction performance for anthocyanins. The best extraction efficiency was obtained with choline chloride:proline:malic acid. Anthocyanins were separated using macroporous resin Amberlite XAD-16 and were eluted with 96% ethanol. Ethanol eluents were valorised by testing their
biological activity in in vitro assay using three cell lines (HeLa, MCF-7, and HEK293T), and the most evident toxic effect was caused by betaine:malic acid. Antioxidant activity of the ethanol eluents was also determined, and the highest value obtained for the betaine:malic acid. The developed procedures based on extraction and direct macroporous resin enrichment processes could be an alternative and environmental method for the extraction and enrichment of anthocyanins from grape pomace.

• Keywords: eutektična otapala; zelena ekstrakcija; antocijani; makroporozna smola; in vitro biološka aktivnost
• Keywords (english): deep eutectic solvents; green extraction; anthocyanins; macroporus resin; in vitro biological activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:259586
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-26 07:37:24