In vitro biološka aktivnost ferocenskih peptidomimetika

undergraduate thesis

• Title (english): In vitro biological activity of ferrocene peptidomimetics
• Author: Anamaria Šintić
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2019-06-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kako bi se ispitala biološka aktivnost i različita svojstva spojeva dobivenih kemijskom sintezom ili izoliranih iz prirodnih izvora, koriste se in vitro testovi citotoksičnosti na humanim ili životinjskim staničnim linijama. In vitro testovi često su prvi korak u ispitivanju biološki aktivnih spojeva te preliminarna smjernica u procesu stvaranja novih lijekova, čiji učinak se kasnije ispituje primjenom in vivo testova na životinjama. Peptidomimetici se definiraju kao spojevi koji oponašaju biološku aktivnost prirodnih peptida, modificirajući njihovu nativnu konformaciju s ciljem poboljšanja stabilnosti, selektivnosti te funkcionalnosti. U ovom istraživanju provedeno je in vitro ispitivanje djelovanja četiri različita ferocenska peptidomimetika na humanu tumorsku staničnu liniju (HeLa) te je određena vijabilnost stanica MTS metodom. Svi ispitani spojevi peptidomimetika pokazali su inhibitorni učinak na rast i proliferaciju tumorskih HeLa stanica 48 sati nakon tretmana. Također, proveden je test formiranja kolonija, kojim je dokazan inhibitorni učinak ispitanih spojeva i na sposobnost HeLa stanica da tvore kolonije. Dobiveni rezultati ukazuju da ispitani ferocenski peptidomimetici imaju antiproliferativno djelovanje na tumorske HeLa stanice što može ukazati na mogući antitumorski učinak.

Abstract (english)

In vitro test of cytotoxicity on animal and human cell lines are used for examination biological activity and different properties of compounds obtained by chemical synthesis or isolated from natural sources. In vitro tests are often the first step in examination biologically active compounds and the preliminary guideline in the process of creating new drugs. Peptidomimetics mimic the biological activity of natural peptides with improved selectivity, functionality and bioavailability. In this research, biological activity of four different ferrocene peptidomimetics was tested on humane tumor cell line (HeLa) and viability was determined by MTS method. All tested peptidomimetics showed an inhibitory effect on cell growth and proliferation HeLa cell line 48 hours after the treatment. Furthermore, colony forming assay was performed, which demonstrated inhibitory effect on cell ability to form colonies. The obtained results indicate that ferrocene peptidomimetics have antiproliferative activity on tumor cells which may indicate a possible antitumor effect.

• Keywords: citotoksičnost; ferocenski peptidomimetici; HeLa tumorska stanična linija; klonogena analiza; vijabilnost
• Keywords (english): cytotoxicity; clonogenic assay; ferrocene peptidomimetics; HeLa tumor cell line; viability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:763715
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-07-12 08:42:22