Utjecaj ubrzane ekstrakcije otapalima pri povišenom tlaku na udio fenolnih spojeva i antioksidacijski kapacitet u sjemenkama komorača

undergraduate thesis

• Title (english): The impact of pressurized liquid extraction on total phenols, total flavonoids and antioxidant capacity of fennel seeds
• Author: Petra Tonković
• Mentor: Maja Repajić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2019-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Cilj ovog istraživanja bio je optimirati uvjete ubrzane ekstrakcije otapalima pri povišenom tlaku (PLE) za izolaciju ukupnih fenola i ukupnih flavonoida iz odmašćenih sjemenki komorača uzastopnom ekstrakcijom 30 %-tnom vodenom otopinom acetona te 30 %-tnom vodenom otopinom metanola. Također, u svim dobivenim ekstraktima određen je antioksidacijski kapacitet. Koncentracije ukupnih fenola u acetonskim ekstraktima određene su u rasponu od 302,66 - 469,10 mg/100 g, a u metanolnim ekstraktima od 87,19 - 107,00 mg/100 g. Najveći prinosi ukupnih fenola uz 30 %-tni aceton kao ekstrakcijsko otapalo dobiveni su pri 100 oC/3 ciklusa, a uz 30 %-tni metanol pri 100 oC/1 ciklus. Koncentracije ukupnih flavonoida u acetonskim ekstraktima određene su u rasponu od 41,90 - 76,90 mg/100 g, a u metanolnim ekstraktima od 12,78 - 19,01 mg/100 g. Najveći prinosi ukupnih flavonoida dobiveni su pri 100 oC/3 ciklusa u ekstrakciji s oba otapala. Znatno viši antioksidacijski kapacitet određen je u acetonskim ekstraktima, gdje se ujedno pokazala vrlo visoka korelacija između antioksidacijskog kapaciteta i udjela ukupnih fenola te ukupnih flavonoida.

Abstract (english)

The aim of this study was to optimize the conditions of pressurized liquid extraction (PLE) for total phenols and total flavonoides isolation from defatted fennel seeds by successive extraction with 30 % aqueous acetone solution and 30 % aqueous methanol solution. Furthermore, antioxidant capacity was determined in all obtained extracts. Concentrations of total phenols in acetone extracts were determined in the range of 302.66 – 469.10 mg/100 g and in methanol extracts they were in range of 87.19 – 107.00 mg/100 g. The highest yields of total phenols with 30 % acetone solution as extraction solvent were obtained at 100 oC/3 cycles and with 30 % methanol solution at 100 oC/1 cycle. Concentrations of total flavonoids in acetone extracts were determined in the range of 41.90 – 76.90 mg/100 g and in methanol extracts they ranged from 12.78 – 19.01 mg/100 g. The highest yields of total flavonoids were obtained at 100 oC/3 cycles by extraction with both solvents. Considerable higher antioxidant capacity was determined in acetone extracts, where very strong correlation between the antioxidant capacity and content of total phenols as well as total flavonoid content was observed.

• Keywords: antioksidacijski kapacitet; fenoli; flavonoidi; komorač; PLE
• Keywords (english): antioxidant capacity; fennel; flavonoids; phenols; PLE
• Language: croatian
• URN:NBN: urn:nbn:hr:159:166674
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-07-26 08:13:15