Nove metode izolacije i detekcije biološki aktivnih spojeva iz ulja i pogače lana

master's thesis

• Title (english): Application of new technologies in isolation and determination of biological active compounds from flaxseed oil and flaxseed cake
• Author: Paula Radonić
• Mentor: Marko Obranović (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Oil and Fat Technology); Zagreb
• Defense date and country: 2018-11-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Lan obiluje ciklolinopeptidima (CLP), lignanima i fenolnim kiselinama. CLP biološki su aktivni spojevi i nosioci gorkog okusa ulja, što je rezultat oksidacije. Cilj ovog rada bio je pojednostavniti proces ekstrakcije primjenom mikrovalnog zračenja te ispitati učinkovitost i mogućnost zamjene metanola s ekološki prihvatljivijim eutektičkim otapalima (DES). Također cilj bio je detektirati i izolirati lignan sekoizolarikirezinol-diglikozid (SDG) te glukozide p-kumarinske i ferulinske kiseline iz pogače lana primjenom tankoslojne kromatografije (TLC). Najveća koncentracija CLE’(49.2 μg g-1), E (2001.8 μg g-1) i B (315.9μg g-1) postignuta je pri mikrovalnom zračenju (200 W, 6 minuta), a najveća koncentracija CLA (2752.9 μg g-1) postignuta je tijekom 8 minuta. Od ispitivanih DES najpogodnije otapalo za ekstrakciju ciklolinopeptida su: 90% kolin-klorid-etilen-glikol (CLE 1643.7 μg g-1) i 90% kolin-klorid-urea-etilen-glikol (CLA 701.5 μg g-1), a za ekstrakciju CLB je 80% kolin-klorid-glukoza (362.8 μg g-1). SDG je uspješno izoliran TLC upotrebom sustava metanol/etilacetat 1.5:1, dok glukozide p-kumarinske i ferulinske kiselina nije bilo moguće razdvojiti TLC metodom.

Abstract (english)

Flaxseed is rich withcyclolinopeptides (CLP), lignans and phenolic acids. CLP show biological activity but they tend to oxidize and influence bitter taste in oil. The aim of this paper was to simplify the extraction process using microwave extraction, to examine the efficiency and the possibility of replacing methanol with green eutectic solvents (DES) and to detect, isolate lignan secoisolariciresinol diglucoside (SDG) andglucosides of p-coumarin and ferulic acid using thin layer chromatography (TLC). The highest concentrations of CLE’(49.2 μg g-1), E (2001.8 μg g-1) and B (315.9 μg g-1) were achieved at 200 W during 6 minutes. The best CLA (2752,9 μg g-1) extraction was during 8 minutes. Suitable DES for cyclolipeptide extraction were 90% choline chloride ethylene glycol for CLE (1643.7 μg g-1), 90% choline chloride urea ethylene glycol for CLA (701.5 μg g-1) and 80% choline chloride glucose (362.8 μg g-1) for CLB extraction. SDG was successfully isolated by TLC using methanol/ethyl acetate 1.5:1, while the glucosides of p-coumarin and ferulic acid were not .

• Keywords: laneno ulje; lanena pogača; ciklolinopeptidi; fenolne kiseline; eutektička otapala; mikrovalna ekstrakcija
• Keywords (english): flaxseed cake; flaxseed oil; cyclolinopeptides; phenolic acids; deep eutectic solvents; ultrasonic – microwave assisted extraction
• Language: croatian
• URN:NBN: urn:nbn:hr:159:053487
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-11-14 12:02:18