Utjecaj omjera C/P na biološko uklanjanje fosfora u anoksično/aerobnom procesu

master's thesis

• Title (english): The effect of C/P ratio on biological phosphorus removal in anoxic/oxic process
• Author: Toni Šipalo
• Mentor: Tibela Landeka Dragičević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for the Biological Waste Water Treatment); Zagreb
• Defense date and country: 2018-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Poboljšano biološko uklanjanje fosfora (EBPR) je važna strategija u spriječavanju eutrofikacije. U EBPR procesu fosfat akumulirajući organizmi (PAOs) uklanjaju P više od svojih anaboličkih zahtjeva unutarstaničnim akumuliranjem u obliku polifosfata (poliP). U anoksično/aerobnom procesu kapacitet i potencijal PAOs za uklanjanje P je istražen pri različitim omjerima C/P (1, 3, 5, 7, 10). Natrijev acetat je uporabljen kao jedini izvor ugljika. Anoksično/aerobni EBPR proces rezultira simultanim uklanjanjem C, N i P, s konfiguracijom: 2 sata anoksičnog procesa (unutarstanično pohranjivanje PHA u PAOs, nema otpuštanja PO4-P) i, u slijedu aerobni proces (aktivnost PAOs –> unutarstanično nagomilavanje PO4-P u obliku poliP); aktivnost nitrifikanata i denitrifikanata (NI/DNI) –> istovremena nitrifikacija i denitrifikacija, i aktivnost heterotrofnih organizama (OHO) –> uklanjanje KPK preostalog nakon anoksične obrade pri C/P≥3). Povećanje omjera C/P rezultira povećanjem anoksičnog unosa KPK i aerobnog unosa P. Neto uzimanje PO4-P iznosi 4,86 mgPO4-P/gVSS (pri C/P 10) i 3,61 mgPO4-P/gVSS (pri C/P 1). FISH metodom je dokazan mikrobni klaster odgovoran za uklanjanje P (PAOs). Također, dokazan je unutarstanično pohranjen poliP.

Abstract (english)

Enhanced biological phosphorus removal (EBPR) is an important strategy in preventing eutrophication. In EBPR phosphate accumulating organisms (PAOs) remove phosphorus beyond its anabolic requirements by accumulating intracellular polyphosphates (polyP). In an anoxic/oxic process the capacities and potential of PAOs for P removal was investigated at different C/P (1, 3, 5, 7, 10). Sodium acetate was used as the sole source of carbon. The anoxic/aerobic EBPR process resulted in a simultaneous removal of C, N and P, with the following configuration: 2h anoxic phase (intracellular PHA storage in PAOs; no release of PO4-P), followed by aerobic phase (PAOs activity –> intracellular PO4-P accumulation in the form of polyP; nitrifiers/denitrifiers (NI/DNI) activity –> simultaneous nitrification denitrification (SND) and, ordinary heterotrophic organisms (OHO) activity –> removal of COD remaining after the anoxic phase in experiments at C/P≥3). The increase in the C/P ratio results in an increase in anoxic intake of COD and aerobic intake of P. The net intake of PO4-P was 4.86 mgPO4-P/gVSS (at C/P 10) and 3.61 mgP/gVSS (at C/P 1). Using the FISH method, a microbial cluster responsible for P removal (PAOs) was proven. Also, intracellular stored polyP was proven.

• Keywords: poboljšano biološko uklanjanje fosofra (EBPR); anoksično/aerobno; PAOs; istovremena nitrifikacija i denitrifikacija; C; N and P removal
• Keywords (english): enhanced biological phosphorus removal (EBPR); anoxic/aerobic; PAOs; simultaneous nitrification; denitrification
• Language: croatian
• URN:NBN: urn:nbn:hr:159:519108
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-26 13:26:28