Učinak proteinskog izolata iz uljne pogače lana na rast i produktivnost kulture životinjskih stanica

master's thesis

• Title (english): Effect of protein isolates from flaxseed meal on animal cell culture growth and productivity
• Author: Marijan Logarušić
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2018-04-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Mnoge industrije sve više teže za efikasnijim iskorištavanjem organskih ostataka nastalih nakon prerade proizvoda iz različitih grana poljoprivrede i proizvodnje hrane. Kao jedan od takvih organskih ostataka s velikim potencijalom primjene ističe se uljna pogača koja zaostaje nakon ekstrakcije ulja. Pogače lana, zbog svog sastava proteina, predstavljaju potencijalnu sirovinu za primjenu u tehnologiji životinjskih stanica. Stoga je u ovom radu ispitan utjecaj proteinskih izolata iz uljne pogače lana na rast i produktivnost CHO-RP stanične linije koja proizvodi rekombinantni protein. Također, ispitan je utjecaj proteinskih izolata i na rast HEK-293T stanične linije. Budući da serum, kao standardni nutritivni suplement u hranjivom mediju, ima određenih nedostataka, istražena je mogućnost korištenja proteinskih izolata kao potencijalna zamjena za serum. Istraživanje je pokazalo da dodatak proteinskih izolata u koncentracijama od 0,1 g L-1 i 0,2 g L-1 u hranjivi medij ima pozitivan učinak na rast i produktivnost CHO-RP stanica, dok u koncentracijama većim od 1 g L-1 dolazi do inhibicijskog djelovanja na rast obje stanične linije. Također je dokazano da se proteinski izolati uljne pogače lana ne mogu koristiti kao djelomična zamjena za serum jer nisu pokazali utjecaj na rast stanica u mediju s 1% seruma.

Abstract (english)

A lot of industries are looking for efficient exploitation of organic residues from various domain of agriculture and food manufacturing. Oil meal, a substance that remains after oil extraction from seeds, is one of these organic residues with great potential of application. Because of its protein content, the flaxseed meal can potentionally be used in animal cell technology. Therefore, in this work, the influence of protein isolates from flaxseed meal on growth and productivity of a recombinant protein producing cell line (CHO-RP) was investigated. The effect of protein isolates on growth of HEK-293T cell line was observed as well. Since the serum, as a standard nutritional supplement in culture media, has certain disadvantages, protein isolates were investigated as possible serum replacement. The results of the research have shown that the addition of protein isolates at concentrations of 0.1 g L-1 and 0.2 g L-1 in cell culture medium has positive effect on growth and productivity of CHO-RP cells. However, when concentration of protein isolates in medium is above 1 g L-1 there is an inhibitory effect on cell growth. Also, it has been proven that protein isolates from flaxseed meals cannot be used as a partial substitution for serum because they have not shown the effect on cell growth in 1% serum media.

• Keywords: uljna pogača lana; proteinski izolat; rekombinantni protein; kulture životinjskih stanica
• Keywords (english): flaxseed meal; protein isolate; cell culture recombinant protein; animal cell culture
• Language: croatian
• URN:NBN: urn:nbn:hr:159:415160
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-28 10:00:26