Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku

master's thesis

• Title (english): Isolation of pigments from algae Chlorella vulgaris using accelerated solvent extraction
• Author: Niki Simonović
• Mentor: Verica Dragović Uzelac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2018-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj ovog istraživanja bio je utvrditi utjecaj broja ciklusa (1, 2), temperature (100, 130, 180 °C) i vremena (5, 10, 15 min) na prinos klorofila i karotenoida ekstrahiranih iz zelene alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima uz povišeni tlak (PLE), uz etanol kao ekstrakcijsko otapalo. Nadalje, cilj je bio optimirati parametre PLE pri kojima će se ostvariti najveći prinosi klorofila i karotenoida. Optimalni uvjeti ekstrakcije klorofila a postignuti su pri temperaturi 160 °C, vremenu ekstrakcije 12 minuta kroz 2 ekstrakcijska ciklusa, a za klorofil b pri 130 °C, 9 minuta i 1 ekstrakcijski ciklus. Optimalni uvjeti pri kojima se dobivaju veći prinosi karotenoida su temperatura 140 °C, vrijeme ekstrakcije 5 minuta i 2 ekstrakcijska ciklusa. Masena koncentracija klorofila a pri optimalnim uvjetima iznosi 14,11 mg g-1 s.t., klorofila b 5,69 mg g-1 s.t., a karotenoida 2,15 mg g-1 s.t. Na temelju statističke analize MANOVA utvrđeno je da svi istraživani parametri imaju značajan utjecaj na prinose istraživanih pigmenata. Pomoću regresijskih modela određene su predviđene vrijednosti pigmenata u ekstraktu koje su eksperimentalno potvrđene.

Abstract (english)

The aim of this study was to determine the influence of number of cycles (1, 2), temperature (100, 130, 180 ° C) and time (5, 10, 15 min) on the yield of chlorophyll and carotenoid extracted from green algae Chlorella vulgaris by accelerated extraction with elevated pressure (PLE), with ethanol as an extraction solvent. Furthermore, the aim was to optimize PLE parameters, with the highest yields of chlorophyll and carotenoid. Optimal conditions of chlorophyll extraction were achieved at 160 ° C, 12 min extraction time for 2 extraction cycles and for chlorophyll b at 130 ° C for 9 minutes and 1 extraction cycle. The optimal conditions for obtaining higher carotenoid yields are 140 ° C, 5 min extraction time and 2 extraction cycles. The mass concentration of chlorophyll at optimal conditions was 14.11 mg g-1 d.m., chlorophyll b 5.69 mg g-1 d.m., and carotenoid 2.15 mg g-1 d.m. Based on the MANOVA statistical analysis, all the parameters investigated have a significant effect on the yields of the investigated pigments. Regression models provided predicted values of pigments in the extract that were experimentally verified.

• Keywords: alge; pigmenti; klorofili; karotenoidi; ekstrakcija; PLE
• Keywords (english): algae; pigments; chlorophylls; carotenoids; extraction; PLE
• Language: croatian
• URN:NBN: urn:nbn:hr:159:195623
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-02 14:27:56