Djelovanje proteinskog hidrolizata iz uljne pogače lana na rast i produktivnost CHO DP-12 stanica

master's thesis

• Title (english): Effect of flaxseed cake protein hydrolysate on growth and productivity of CHO DP-12 cells
• Author: Sara Berljavac
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2019-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Posljednjih desetljeća uočeni su rizici vezani uz korištenje seruma u proizvodnji terapeutika pomoću kultura životinjskih stanica, pa se sve više nastoje razviti mediji bez dodatka seruma. Hidrolizati osiguravaju važne nutrijente stanicama, a osim što mogu potaknuti rast staničnih linija, istraživanja su pokazala da pozitivno utječu i na produktivnost staničnih linija. Cilj ovog rada bio je ispitati učinak proteinskih hidrolizata, dobivenih pomoću enzima Alcalase, Neutrase i Protamex, uljne pogače lana na rast i produktivnost stanične linije CHO DP-12, proizvođača humanog protutijela imunoglobulina (IgG) anti IL-8. Također, istražena je i mogućnost zamjene dijela seruma proizvedenim hidrolizatima. Istraživanje je pokazalo da sva tri proteinska hidrolizata pozitivno djeluju na rast stanica, no samo proteinski hidrolizat dobiven pomoću Alcalase ima statistički značajan pozitivan učinak na produktivnost CHO DP-12 stanične linije. Hidrolizati lana mogu se koristiti kao djelomična zamjena za serum s obzirom da je dodatkom i najmanjeg volumnog udjela hidrolizata postignut dobar stanični rast.

Abstract (english)

Scientists are trying to develop a serum free medium because of the risks associated with the use of serum in the production od therapeutics using animal cell cultures. Hydrolysates provide important nutrients to the cells and cause growth stimulation and positive effect on the productivity of cell lines. The aim of this study was to investigate the effect of protein hydrolysates from flaxseed cake obtained by the Alcalase, Neutrase and Protamex enzymes on the growth and productivity of CHO DP-12 cell lines, producers of immunoglobulin (IgG) anti IL-8. Also, the possibility of replacing part of serum with hydrolysates was investigated. Research has shown that all protein hydrolysates positively affect cell growth but only the protein hydrolysate obtained with Alcalase has a statistically significant positive effect on the productivity. Flax hydrolysates can be used as a partial substitution for serum since a good cell growth has been achieved by addition of the smallest volume of hydrolysates.

• Keywords: proteinski hidrolizati; monoklonsko protutijelo; CHO DP-12 stanična linija; uljna pogača lana
• Keywords (english): protein hydrolysate; monoclonal antibody; CHO DP-12 cell line; flaxseed cake
• Language: croatian
• URN:NBN: urn:nbn:hr:159:859177
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-12-05 10:27:16