Izolacija pigmenata iz alge Cystoseira sp. primjenom različitih tehnika ekstrakcije

master's thesis

• Title (english): Isolation of pigments from algae Cystoseira sp. applying of different extraction techniques
• Author: Antonia Jujnović
• Mentor: Verica Dragović Uzelac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for drying Technologies and monitoring of biologically active compounds); Zagreb
• Defense date and country: 2019-03-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cystoseira je vrsta smeđe alge koja spada u red Fucales, te predstavlja najznačajniju i najrasprostranjeniju smeđu algu Jadranskog mora, te je izvor raznih skupina bioaktivnih molekula. U ovom radu ispitan je utjecaj različitih tehnika ekstrakcije (ekstrakcija pri sobnoj temperaturi uz kontinuirano miješanje te vrtložno miješanje uz djelovanje centrifugalne sile i ekstrakcija pomoću ultrazvučne kupelji), vremena ekstrakcije (5, 10 i 15 minuta) te otapala (aceton 80%, metanol i etanol) na prinos pigmenata (klorofili, karotenoidi) alge Cystoseira. Maseni udjeli pigmenata (klorofila-a, klorofila-b i ukupnih karotenoida) određeni su primjenom spektrofotometrijskih metoda. Najbolji prinosi pigmenata dobiveni su u ekstraktima dobivenim u ultrazvučnoj kupelji, a kao najbolje otapalo za ekstrakciju karotenoida i klorofila-a pokazao se 80% aceton, dok su nešto bolji prinosi klorofila-b dobiveni s etanolom. Optimalno vrijeme ekstrakcije u ultrazvučnoj kupelji je 10 min za karotenoide, 11 min za klorofil-a i 14 min za klorofil-b.

Abstract (english)

Cystoseira is a type of brown alga that belong to the order of Fucales, and represents the most significant and most widespread brown algae of the Adriatic Sea and also a source of various groups of bioactive molecules. In this thesis, the influence of different extraction techniques (extraction at room temperature with continuous stirring, vortexing assisted by the centrifugal force and ultrasonic bath extraction), extraction time (5, 10 and 15 minutes) and solvent (acetone 80%, methanol and ethanol) were examined to determine yield of pigments (chlorophylls and carotenoids) from algae Cystoseira. Mass fractions of pigments (chlorophyll-a, chlorophyll-b and total carotenoids) were determined by spectrophotometry method. Ultrasonic bath gave the biggest yield of pigments, and the most preferred solvent for carotenoid and chlorophyll-a extraction is 80% acetone, although for chlorophyll-b extraction ethanol was better solvent. Optimal extraction time in the ultrasonic bath is 10 min for carotenoids, 11 min for chlorophyll-a and 14 min for chlorophyll-b.

• Keywords: Cystoseira; ekstrakcija; pigmenti; otapalo; spektormetrija
• Keywords (english): Cystoseira; extraction; pigments; solvent; spectrophotometry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:525152
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-10 13:25:22