Biološki učinak polifenola iz mahune graška i ljuske luka kao sekundarnih izvora fitokemikalija

master's thesis

• Title (english): Biological effect of polyphenols from pea peel and onion skin as a secondary source of phytochemicals
• Author: Željka Stanečić
• Mentor: Ksenija Durgo (mentor)
• Committee member: Draženka Komes (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2020-11-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Prehrambena industrija proizvodi veliku količinu otpadne biomase koja predstavlja ekološki rizik, stoga se danas intenzivno istražuju novi načini valorizacije otpadnih materijala. Nusproizvodi poput mahune graška i ljuske luka nedovoljno su iskorišteni izvori bioaktivnih spojeva, među kojima se ističu polifenoli, sekundarni biljni metaboliti koji posjeduju visok antioksidacijski potencijal te pružaju značajnu zaštitu od razvoja raznih kroničnih bolesti. Cilj rada je istražiti biološki učinak ekstrakata mahune graška i ljuske luka na stanične makromolekule te humane stanične linije karcinoma epitela usne šupljine Cal27 i hepatocelularnog karcinoma jetre HepG2 u koncentracijama pri kojima se ovi spojevi mogu naći u realnim uvjetima u probavnom sustavu čovjeka. Rezultati su pokazali da polifenoli sadržani u mahuni graška djeluju protektivno na stanične makromolekule sprečavajući štetne učinke UV zračenja i slobodnih radikala na genetički materijal te oksidacijske promjene na proteinima i lipidima, dok je ljuska luka bogata polifenolima koji u određenim uvjetima mogu ispoljiti prooksidacijsko i genotoksično djelovanje

Abstract (english)

The food industry produces a large amount of waste biomass that poses as an environmental risk, hence today new ways of valorizing waste materials are being intensively researched. By-products such as pea peel and onion skin are underutilized sources of bioactive compounds, such as polyphenols, secondary plant metabolites that have high antioxidant potential and provide significant protection against the development of various chronic diseases. The aim of this study is to determine the biological effect of pea peel and onion skin extracts on cellular macromolecules, human oral epithelial carcinoma cell line Cal27 and hepatocellular liver carcinoma cell line HepG2 in concentrations at which these compounds can be found in the human digestive system. The results showed that polyphenols contained in the pea peel have a protective effect on cellular macromolecules by preventing the harmful effects of UV radiation and free radicals on genetic material and oxidative changes on proteins and lipids, while onion skin is rich in polyphenols that can exert prooxidative and genotoxic effects.

• Keywords: sekundarni izvori polifenola; antioksidacijsko djelovanje; genotoksičnost; stanične makromolekule; stanične linije karcinoma epitela usne šupljine (Cal27) i hepatokarcinoma (HepG2)
• Keywords (english): secondary sources of polyphenols; antioxidant effect; genotoxicity; cellular macromolecules; oral epithelial carcinoma (Cal27); and hepatocarcinoma (HepG2) cell lines
• Language: croatian
• URN:NBN: urn:nbn:hr:159:647177
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-12-08 14:06:05